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TranscriptomicsXenopus M-phase extract treated induced pluripotent stem cells
Transfer of somatic cell nuclei to enucleated eggs or ectopic expression of specific transcription factors are two different reprogramming strategies used to generate pluripotent cells from differentiated cells. However, they are poorly efficient and other unknown factors might be required to increa...
ORGANISM(S): Mus musculus 
2011-11-21 | E-GEOD-31691 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation to identify CUG-BP1/EDEN-BP target mRNAs in Xenopus tropicalis eggs
Identification of RNA bound to the RNA-binding protein CUGBP1/EDENBP in Xenopus tropicalis eggs.
ORGANISM(S): Xenopus tropicalis 
2008-02-21 | E-MEXP-1466 | biostudies-arrayexpress
TranscriptomicsLocalization profiling of mRNAs associated with microtubules and microtubule bound polysomes in human HeLa cells and unfertilized Xenopus eggs demonstrates conserved localization of mRNAs to mitotic microtubules
Microtubules were purified from mitotic Xenopus or Hela extracts and associated mRNAs were compared to total extract.
ORGANISM(S): Homo sapiens 
2007-12-31 | E-MEXP-1320 | biostudies-arrayexpress
ProteomicsIdentification of Yap-interacting proteins in Xenopus egg extracts by co-immunoprecipitation coupled to LC/MS/MS
Yap (Yes-associated protein 1) is a downstream effector of the Hippo signaling pathway and known as a transcriptional co-activator. We previously identified Yap1 as a novel factor implicated in the control of the temporal DNA replication program in Xenopus retinal stem cells. However, whether Yap is...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2022-08-09 | PXD029345 | Pride
Lc-msms Xenopus laevis Ip 
TranscriptomicsOct3/4 expression and notch signaling in bone marrow stromal cells reprogrammed by Xenopus egg extract
Bone marrow stromal cells (BMSCs) are multipotent stem cells that preferentially differentiate into mesenchymal cells. If they can be dedifferentiated into embryonic stem cell-like cells, they will be a highly attractive source for cell therapy. Cell and egg extracts have been used in a few studies ...
ORGANISM(S): Mus musculus 
2008-07-11 | E-GEOD-11660 | biostudies-arrayexpress
TranscriptomicsXenopus egg small RNA associated with Y12 antibody
We examined in Xenopus tropicalis eggs piRNAs that are associated with Y12 antibody, which binds symmetrically methylated arginines that are present on diverse Piwi proteins. The goal of this experiment is to more deeply characterize the piRNA pool from adult Xenopus extracts, using the Illumina pla...
ORGANISM(S): Xenopus (Silurana) tropicalis 
2010-01-04 | E-GEOD-19173 | biostudies-arrayexpress
ProteomicsPurified native gamma-tubulin ring complex from Xenopus laevis egg extracts
Native gamma-tubulin ring complex from Xenopus laevis egg extracts purified as used for cryo-electron microscopy
ORGANISM(S): Xenopus laevis (African clawed frog) 
2025-03-13 | PXD060119 | Pride
Nedd1 Microtubule Nucleation Laevis Gamma-tubulin Gcp3 Xenopus Gcp2 Gamma-tubulin ring complex Gcp6 Gcp5 Gcp4 Actin Mzt1 Mzt2 
ProteomicsDNA replication regulates SPRTN- and Proteasome-dependent DPC proteolysis
DNA-protein crosslinks (DPCs) are bulky DNA lesions that interfere with DNA metabolism and therefore threaten genomic integrity. Recent studies implicate the metalloprotease SPRTN in S-phase removal of DPCs, but how SPRTN activity is coupled to DNA replication is unknown. Using Xenopus egg e...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-02-13 | PXD008831 | Pride
Plasmid pull-down Pp-ms Dpc repair Dna repair Xenopus egg extract Dna replication 
ProteomicsDIA analysis if of X. laevis egg extracts
Mass spectrometry DIA analysis of MG132 treated Xenopus laevis egg extracts and compared with control (untreated) extracts to identify changes in protein levels upon MG132 treatment.
ORGANISM(S): Xenopus Laevis (ncbitaxon:8355) 
2024-11-03 | MSV000096280 | MassIVE
ProteomicsReplisome composition of stalled, terminating replication forks
Topological stress can cause replication forks to stall as they converge upon one another during termination of vertebrate DNA synthesis. However, replication forks ultimately overcome topological stress and complete DNA synthesis, suggesting that alternative mechanisms can overcome topological stre...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2023-02-21 | PXD031170 | Pride
Xenopus Lc-msms Topoisomerase Egg extracts Termination Dna replication 
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