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ProteomicsMass spectrometry analysis of in vitro PKCe phosphorylated ADSL
Bacterially purified His-ADSL proteins on Ni-NTA agarose beads were incubated with or without active PKCε in the presence of ATP for an in vitro kinase assay. After centrifugation, the agarose beads were collected and mixed with 30 μL of SDS-PAGE sample loading buffer. The samples were then separate...
ORGANISM(S): Homo sapiens (Human) 
2026-02-16 | PXD074509 | Pride
In vitro kinase assay  lc-ms/ms  phosphorylation 
ProteomicsImmunoprecipitation of INSIG1/2 and mass spectrometry analysis
Huh7 cells with or without expression of Flag-INSIG1/2 were treated with or without high glucose for 2 h. An immunoprecipitation assay was performed using anti-Flag antibody, and immunoprecipitates of Flag-INSIG1 were eluted with Flag peptide, separated using SDS-PAGE and stained with Coomassie Bril...
ORGANISM(S): Homo sapiens (Human) 
2025-12-26 | PXD054753 | Pride
Insig1/2 mass spectrometry differntial proteins analysis 
ProteomicsHCC INSIG1 Mass Spectrometric Analysis
Following high-glucose treatment of approximately 2 × 10⁸ Huh7 cells, immunoprecipitation was performed using an anti-INSIG1 antibody. Subsequent mass spectrometric analysis of a tryptic fragment identified succination at C167.
ORGANISM(S): Homo sapiens (Human) 
2025-12-26 | PXD071572 | Pride
Hcc cell lines Lc–ms/ms 
ProteomicsPSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation
MDA-MB-231 cells with expression of Flag-GPX4 were treated with IFNg. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.
ORGANISM(S): Homo Sapiens 
2025-03-01 | PXD061373 |
ProteomicsGPX4 immunoprecipitation in Huh7 cells
Huh7 cells with expression of Flag-GPX4 were treated with IGF1. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.
ORGANISM(S): Human 
2023-02-22 | MSV000091343 | MassIVE
ProteomicsIdentification of SNHG9-binding proteins in Huh7 cells using RNA pull-down assay
This project aims to identify the specific proteins interacting with lncRNA SNHG9 in hepatoma cells. We performed RNA pull-down assays using biotin-labeled SNHG9 probes in Huh7 cell lysates, followed by mass spectrometry (LC-MS/MS) to analyze the pulled-down protein.
ORGANISM(S): Homo Sapiens 
2026-04-20 | PXD077375 |
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