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Mass spectrometry analysis of in vitro PKCe phosphorylated ADSL

ABSTRACT: Bacterially purified His-ADSL proteins on Ni-NTA agarose beads were incubated with or without active PKCε in the presence of ATP for an in vitro kinase assay. After centrifugation, the agarose beads were collected and mixed with 30 μL of SDS-PAGE sample loading buffer. The samples were then separated by SDS-PAGE and visualized by Coomassie Brilliant Blue staining. The protein bands were excised from the gel, digested with trypsin, and analyzed by LC-MS/MS using an Orbitrap Elite mass spectrometer.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: yuran duan

LAB HEAD: Daqian Xu

PROVIDER: PXD074509 | Pride | 2026-02-16

REPOSITORIES: Pride

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			Action	DRS
	Q4_FWMS20242501_1.msf	Msf
	Q4_FWMS20242501_1.raw	Raw
	checksum.txt	Txt

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