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Proteomicsm6A binding proteins identified by SPIDER assay
To assess whether SPIDER could identify modified nucleic acids binding proteins within a complex environment, such as the cellular milieu, we sought to identify N6-methyladenosine (m6A) binding proteins within a total cell lysate. We performed SPIDER assay by incubating biotin-ssRNAs（with and withou...
ORGANISM(S): Homo Sapiens 
2022-11-02 | PXD026519 |
ProteomicsLenalidomide binding proteins identified by SPIDER assay
To assess whether SPIDER could capture protein-small molecule interaction in a complex environment, here we carried out the SPIDER assay by incubating biotin-Lenalidomide or a biotin control with HEK293T total lysate, followed by the use of biotin conjugated beads coupled with mass spectrometry to i...
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026523 |
ProteomicsPupylation sites on protein Sox2
As a test for the effectiveness of SPIDER with RNA-protein pairs, We used Sox2 and specific biotin-DNA and biotin-RNA to validate the efficiency of SPIDER capturing protein-nucleic acid interaction. Pupylation sites on Sox2 after SPIDER assay was identified by LC-MS/MS analysis, by searching for an ...
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026517 |
ProteomicsPupylation sites on SARS-CoV-2 N
As a test for the effectiveness of SPIDER with RNA-protein pairs, we examined SARS-CoV-2 Nucleoprotein. We used Nucleoprotein and specific biotin-RNA to validate the efficiency of spider capturing N and analysised the pupylation sites of N protein by mass spectrometry
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2 
2021-06-04 | PXD026518 |
ProteomicsmRNA-protein interactome of THP-1 cells
To investigate the effectiveness of SPIDER identifying the mRNA interactome, we carried out the SPIDER assay by incubating biotin-oligo(dT) or biotin control directly with cell lysates of THP-1 cells
ORGANISM(S): Homo Sapiens 
2021-06-04 | PXD026521 |
Proteomicsm6A binding proteins identidified by RNA pull down.
Biotinyted nucleic acid probe assay for protein-m6A interaction
ORGANISM(S): Escherichia Coli 
2022-11-02 | PXD037881 |
ProteomicsIdentification of E. coli CheZ interactors by AP-MS
To identify E. coli CheZ interactors, affinity purification (AP) coupled with mass spectrometry (MS) was applied.
ORGANISM(S): Escherichia Coli 
2022-11-04 | PXD037953 |
ProteomicsPupylation sites on protein GFP-PupE
Characterization of GFP-Pup after Pupylation assays by mass spectrometry.
ORGANISM(S): Mycobacterium Tuberculosis H37rv_2009 
PXD026478 |
ProteomicsCheA pupylation sites identified by SPIDER assay
We used CheAs and biotin-CheZ to validate the efficiency of SPIDER capturing protein-protein interaction. Pupylation sites on WT CheAs after SPIDER assay was identified by LC-MS/MS analysis, by searching for an additional mass of ~243 Da, which represents the three C-terminal residues of Pup, i. e.,...
ORGANISM(S): Escherichia Coli 
PXD026509 |
ProteomicsSide-by-side comparison of AP-MS, Tri-functional affinity probe, BioID, PUP-IT and SPIDER to identify CheZ-interacting proteins
Comparison of CheZ interactors’ ratio of two replicate experiments using five different methods, i.e., Affinity-Purification, Tri-functional affinity probe Sulfo-SBED and proximity labeling methods BioID, SPIDER, PUP-IT
ORGANISM(S): Escherichia Coli 
2022-11-03 | PXD037952 |
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