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To identify the circNEIL3 interacting protein, we performed RNA pull-down assays after transfecting into cells with biotin-labeled sense (S) or antisense (AS) DNA oligomers crossing the junction site of circNEIL3, and the pull-down proteins were subjected to SDS-PAGE and Coomassie blue staining. ~45...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039394 | Pride
High blood glucose level is one of the main characteristics of diabetes mellitus (DM). It is speculated that longevity families may have certain advantages in blood glucose regulation. But till now, limited information on these items is reported. In this study, a TMT- based comparative quantitative ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD032932 | Pride
We investigated the potential proteins degraded by E2F4-induced autophagy via quantitative proteomics. To gain insight into protein partner essential for E2F4 stability, co-immunoprecipitation and mass spectrometry assays were performed.
ORGANISM(S): Homo Sapiens 
2021-11-19 | PXD029858 |
Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease. Emphysematous phenotype is a type of most common and critical phenotype, which is characterized by progressive lung destruction and poor prognosis. However, the underlying mechanism of this structural damage has not been...
ORGANISM(S): Homo Sapiens 
2021-01-31 | PXD023900 |
Cell membranes harvested from U251 and primary healthy astrocytes were prepared for LC-MS/MS analysis. After analysis, we could obtain a pool of proteins, which were enriched in the U251 cell membrane sample rather than healthy astrocyte membrane sample.
ORGANISM(S): Homo Sapiens 
2022-01-06 | PXD030760 |
To understand Hashimoto's Thyroiditis and translate this knowledge to clinical applications, we took mass spectrometric analysis on thyroid tissue fine-needle puncture from HT patients and healthy people in an attempt to make a further understanding of the pathogenesis of Hashimoto's Thyroiditis.
ORGANISM(S): Homo Sapiens 
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