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Transcriptomicswenyan
Kashin-Beck disease (KBD) is an endemic and chronic osteochondropathy with unknown etiology. The disease mostly occurs in children between the ages of 3 and 13 in a diagonal belt-like area ranging from Northeast to Southwest China. We carried out this microarray analysis to investigate the differenc...
ORGANISM(S): Homo sapiens 
2014-08-30 | E-MTAB-2494 | biostudies-arrayexpress
ProteomicsDifferent expressed proteins of HIV-infected long-term non-progressors (LTNPs) and regular progressors (RPs)
The whole blood samples were obtained from 3 LTNPs and 2 RPs HIV-1 infected patients, and the CD4+ T cells were isolated using anti-CD4-specific antibody-coated microbeads (130-045-101, Miltenyi Biotec, Germany) according to the manufacturer's instructions. Then the proteins extracted from the CD4+ ...
ORGANISM(S): Homo Sapiens 
2023-10-12 | PXD046078 |
ProteomicsMass spectrometry analysis of VHL and BICD2 binding protein in HEK-293t cells
In order to identify the binding proteins of VHL and BICD2, we enriched their binding proteins by TurboID-based proximity labeling technology and Flag pull-down assay, and performed mass spectrum analysis.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042830 | Pride
Vhl bicd2 ms hek-293t 
GenomicsThe ChIP-Sequence data of STAT92E in Drosophila adult intestines
JAK/STAT pathway plays important roles in controlling Drosophila intestinal homeostasis and regulating the ISC proliferation and differentiation. However,the downstream targets of its transcription factor-STAT92E remain largely unknown.To further identify the regualtory mechanisms of the JAK/STAT pa...
ORGANISM(S): Drosophila melanogaster 
2015-05-12 | E-GEOD-67346 | biostudies-arrayexpress
ProteomicsIdentification of host factors interacting with HIV-1 Tat
The promiscuous biotin ligase TurboID was fused to Tat for precise and temporal biotinylation of surrounding proteins, which could be enriched by streptavidin and identified by mass spectrometry.
ORGANISM(S): Homo Sapiens 
2025-01-03 | PXD059409 |
ProteomicsProteins interacting with SARS-CoV-2 NSP16
HEK293T were transfected with SARS-CoV-2-NSP16-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center fo...
ORGANISM(S): Homo Sapiens 
2024-07-15 | PXD053961 |
ProteomicsHost proteins interacting with EV71-VP2 were identified by mass spectrometry
EV71-VP2-MYC was extransfected into 293T cells, and 36 hours later, the cells were treated with MG132 for 12 hours. The cells were harvested, and the cell lysates were incubated with protein-G with MYC antibody overnight. The interacting proteins were analyzed by mass spectrometry.
ORGANISM(S): Homo Sapiens 
2025-01-07 | PXD059489 |
ProteomicsDifferent expressed proteins of HIV-infected long-term non-progress (LTNPs) and regular progressors (RPs)
To identify potential host factors promoting HIV-1 control and non-progressive infection, we employed mass spectrometry-based proteomic profiling to analyze three LTNPs and two PRs，in order to identify proteins expressed at higher levels in LTNPs.
ORGANISM(S): Homo Sapiens 
2024-05-30 | PXD050294 |
ProteomicsProteins interacting with GBP5 and RSV-SH protein
HEK293T were transfected with RSV-SH-HA, GBP5-HA or VR1012(empty vector) respectively for 48 h and then harvested. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for pr...
ORGANISM(S): Homo Sapiens 
2024-08-06 | PXD054621 |
ProteomicsProteins interacting with SARS-CoV-2 envelope
HEK293T were transfected with SARS-CoV-2-E-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for pr...
ORGANISM(S): Homo Sapiens 
2023-01-01 | PXD039140 |
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