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In order to identify the binding proteins of VHL and BICD2, we enriched their binding proteins by TurboID-based proximity labeling technology and Flag pull-down assay, and performed mass spectrum analysis.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042830 | Pride
HEK293T were transfected with SARS-CoV-2-NSP16-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center fo...
ORGANISM(S): Homo Sapiens 
Targeting transcriptional regulatory complexes represents a promising strategy for activating thermogenic fat and treating obesity. In this study, we focused on identifying critical, specific metabolic pathways enriched during brown adipocyte differentiation. Through analysis of metabolomics and RNA...
2026-03-24 | MTBLS12237 | MetaboLights
HEK293T were transfected with SARS-CoV-2-E-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for pr...
ORGANISM(S): Homo Sapiens 
2023-01-01 | PXD039140 |

Apicomplexan parasite growth and replication relies on nutrient acquisition from host cells, in which intracellular multiplication occurs, yet the mechanisms that underlie the nutrient salvage remain elusive. Numerous ultrastructural studies have documented a plasma membrane invagination with a d...

2023-04-19 | MTBLS7006 | MetaboLights
Stress granules are known to be related with selective translation. To determine which proteins are regulated by stress granules assembly in Ptenα─/─ MEFs, we performed a mass spectrometry analysis of Ptenα+/+ and Ptenα─/─ MEFs under oxidative stress.
ORGANISM(S): Mus Musculus 
PTENα is the first isoform of tumor suppressor PTEN. To investigate the molecular mechanism by which PTENα modulates cell response to inflammation, we performed pull-down assays with FLAG-tagged proteins coupled with mass spectrometry under condition of H2O2 treatment in B16 cells.
ORGANISM(S): Mus Musculus 
2021-07-13 | PXD040709 |
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