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TranscriptomicsDissecting physiological and transcriptional responses of nitric oxide to drought stress by increasing in vivo nitric oxide content in Arabidopsis
Nitric oxide (NO) is involved in all major environmental stresses. However, most of these understandings were mainly based on pharmacological study using NO modulator compounds. Recently, our studies together with others provided a new class of plant experimental system with specific in vivo NO rele...
ORGANISM(S): Arabidopsis thaliana 
2014-06-03 | E-GEOD-48474 | biostudies-arrayexpress
ProteomicsCerebellum PC O-GlcNAcylation TMT analysis
The total protein in the sample was extracted, part of the sample was taken out for protein concentration determination and SDS-PAGE detection, the other part was taken out for trypsin enzymolysis and labeling, and then equal amount of each labeled sample was mixed for chromatographic separation. Fi...
ORGANISM(S): Mus Musculus 
2023-02-09 | PXD040011 |
ProteomicsMulti-omics analysis of human peripheral blood reveals marked molecular profiling changes caused by one night of sleep deprivation
Sleep insufficiency is associated with various disorders (cardiovascular disease, neurodegenerative diseases, immunological disorders.) but the molecular bases for these associations are largely unknown. We used multiple omics techniques to examine changes in the blood of healthy male and female adu...
ORGANISM(S): Homo Sapiens 
2022-04-23 | PXD033402 |
ProteomicsMass spectrometric analysis of fluorophore labeled albumins
A genetic engineering strategy for editing near-infrared-II fluorophores. The labeled albumin proteins were digested using suspension trapping (STrap) approach with in-house packed glass fiber filters. The LC-MS/MS analysis was performed on a nanoLC-Q Exactive MS system. The protein identification w...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-05-07 | MSV000087386 | MassIVE
ProteomicsThe NAE1-mediated Neddylation operates as an essential post-translational modification checkpoint for effector CD8+ T cells
Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,00...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-27 | MSV000094179 | MassIVE
ProteomicsProteomic profiling and tumor microenvironment characterization reveal molecular and immunological hallmarks of left-sided and right-sided colon cancer tumorigenesis
The protein landscapes of LSCC and RSCC were constructed, and the hallmarks of tumorigenesis and characteristics of TME for LSCC and RSCC were identified. These findings improved our understanding of LSCC and RSCC and may facilitate the development of diagnostic or therapeutic targets for LSCC and R...
ORGANISM(S): Homo Sapiens 
2023-06-13 | PXD042962 |
ProteomicsProteome of root from Gossypium barbadense infected by Verticillium
In this study, two kinds of island cotton materials with different resistances of Verticillium dahliae were used for Verticillium dahliae infection, the proteomic was used to detect all the protein expression levels in the roots of the two kinds of cotton, and the spectronaut pulsar x software was s...
ORGANISM(S): Gossypium Barbadense 
2020-05-31 | PXD017527 |
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