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ProteomicsPR1-14A002
In quantitative proteomic assay, isobaric tags for relative and absolute quantitation (iTRAQ) combined with 2D LC-MS/MS is emerging as a powerful methodology in the search for disease specific targets and biomarkers using cell lines, tissues, body fluids and so on. In this study, 5325 distinct prote...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2022-03-02 | PXD004052 | Pride
Sus scrofa  proteinpilot software v4.5 tripletof 5600+ 
ProteomicsSus scrofa (pig) small intestinal mucosa LC-MS/MS
In this study, we applied the isobaric tags for relative and absolute quantitation (iTRAQ) technique to detect alterations in the proteomic profile of the jejunal mucosa using a porcine model in which piglets were offered the protein-limited (PL) diet. Protein identification and quantification for i...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2016-12-22 | PXD004069 | Pride
Jejunal mucosa Pig Lc-ms/ms 
ProteomicsPREDICTIVE MODELING OF THERAPEUTIC RESPONSE TO CHONDROITIN SULFATE/GLUCOSAMINE HYDROCHLORIDE IN KNEE OSTEOARTHRITIS
In the present study we explored potential protein biomarkers useful to predict the therapeutic response of osteoarthritis (OA) patients treated with pharmaceutical grade Chondroitin sulfate/Glucosamine hydrochloride (CS+GH; Droglican, Bioiberica), in order to optimize therapeutic outcomes.
ORGANISM(S): Homo sapiens (Human) 
2019-09-12 | PXD012444 | Pride
Predictive biomarkers Chondroitin sulfate/glucosamine hydrochloride Proteomics Osteoarthritis 
ProteomicsNELF-A phosphorylation site identification
NELF-A phosphorylation by P-TEFb is a key event in the pausing release. We conduct IMAC-LC-MS-MS to identified NELF-A phosphorylation site by activation P-TEFb kinase.
ORGANISM(S): Homo sapiens (Human) 
2016-03-23 | PXD003433 | Pride
Imac Nelf-a Hela cell Human 
ProteomicsMatrisome remodelling during Intervertebral Disc development and ageing
Intervertebral disc (IVD) is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. Although the changes that occur with ageing in the IVD have been under study, no...
ORGANISM(S): Bos taurus (Bovine) 
2017-09-25 | PXD004922 | Pride
Intervertebral disc (ivd)  extracellular matrix (ecm)  nucleus pulpous (np)  itraq 
ProteomicsUse of SWATH mass spectrometry for quantitative proteomic investigation of Shewanella oneidensis MR-1 biofilms grown on graphite cloth electrodes
. In this study we show successful use of SWATH-MS for quantitative proteomic analysis of a microbial electrochemically active biofilm. Shewanella oneidensis MR-1 was grown on carbon cloth electrodes under continuous anodic electrochemical polarizations in a bioelectrochemical system. Using lactate ...
ORGANISM(S): Shewanella oneidensis (strain MR-1) 
2016-08-10 | PXD001472 | Pride
Shewanella oneidensis mr-1 Proteomics Bioelectrochemical system 
ProteomicsHEK-293T LC-MS/MS
We established two in vitro cell-free systems to mimic the activation of mTORC1 and autophagy by monitoring the translocation of two readout proteins Raptor and ATG14. Based on the two systems, we further screened the potential regulators involved in the leucine-mediated mTORC1 and autophagy pathway...
ORGANISM(S): Homo sapiens (Human) 
2022-03-03 | PXD007811 | Pride
Human Cell-free system Lc-ms/ms 
ProteomicsHEK293T LC-MS/MS
Leucine plays crucial roles in protein proteolytic autophagy process, but the underlying mechanisms are not fully understood. Here, we combined in vitro reconstitution with comparative proteomics technology to identify the potential regulators for leucine-mediated autophagy pathway. We established a...
ORGANISM(S): Homo sapiens (Human) 
2022-03-02 | PXD007845 | Pride
Hek293t Cell-free system Leucine Itraq 
ProteomicsProtein idetification by using DNA pull-down & MS assay
Identification of proteins binding to the promoter of hrpR gene in Pseudomonas syringae using DNA pull-down & MS assay
ORGANISM(S): Pseudomonas syringae pv. actinidiae str. Shaanxi_M228 
2021-03-23 | PXD023722 | Pride
Promoter Dna pull-down Lc-ms/ms 
ProteomicsClpP protease modulates bacterial growth, stress response and bacterial virulence in Brucella abortus
Intracellular proteolysis via ATP-dependent proteases is a conserved biological process. The ATP-dependent Clp protease is made up of peptidase ClpP and ATP-dependent chaperones and plays an important role in the proteolysis process. In several pathogenic bacteria, the Clp protease is implicated in ...
ORGANISM(S): Brucella abortus (strain 2308) 
2023-10-24 | PXD034842 | Pride
Virulence Itraq analysis Brucella Clpp protease 
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