HNRNPU RNA targets and DNA methylation changes during neural differentiation

ABSTRACT: This study investigates the molecular mechanisms by which the DNA/RNA-binding protein HNRNPU regulates gene expression during early human neural development. Pathogenic variants in HNRNPU cause a severe neurodevelopmental disorder, but the underlying mechanisms remain poorly understood. To explore the role of HNRNPU in transcriptional and epigenetic regulation, we used human induced pluripotent stem cell (hiPSC)-derived neuroepithelial stem cells (NES) and differentiating neural cells as model systems. We performed formaldehyde crosslinking and ribonucleoprotein immunoprecipitation followed by RNA sequencing (fRIP-seq) to identify RNA targets bound by HNRNPU, whole-genome bisulfite sequencing (WGBS) to assess the impact of HNRNPU silencing on DNA methylation landscapes, and CUT&RUN profili

INSTRUMENT(S): Illumina NovaSeq X, NextSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Marika Oksanen 

PROVIDER: E-MTAB-14910 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress