Carbapenem-resistant Citrobacter freundii harboring bla_KPC-2 and bla_NDM-1: a study on their transferability and potential dissemination via generating a transferrable hybrid plasmid mediated by IS6100.

ABSTRACT:

Introduction

The increase in clinical Enterobacteriaceae with dual carbapenemase has become a serious healthcare concern. It is essential to characterize the transferability and potential dissemination of bla_KPC-2- and bla_NDM-1-coharboring carbapenem-resistant Citrobacter freundii (CRCF).

Methods

Four bla_KPC-2- and bla_NDM-1-coharboring CRCF strains were collected from our surveillance of the prevalence of carbapenem-resistant Enterobacteriaceae. The isolates were assessed using species identification, antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, plasmid stability, and fitness costs. Clonality, genome, plasmidome, and phylogeny were analyzed to re

SUBMITTER: Zhang F 

PROVIDER: S-EPMC10469622 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature