Selective Reduction of Ca²⁺ Entry Through the Human NMDA Receptor: a Quantitative Study by Simultaneous Ca²⁺ and Na⁺ Imaging.

ABSTRACT: Excessive Ca²⁺ influx through N-methyl-D-aspartate type glutamate receptors (NMDAR) is associated with excitotoxicity and neuronal death, but the inhibition of this receptor-channel causes severe adverse effects. Thus, a selective reduction of NMDA-mediated Ca²⁺ entry, leaving unaltered the Na⁺ current, could represent a valid neuroprotective strategy. We developed a new two-fluorophore approach to efficiently assess the Ca²⁺ permeability of ligand-gated ion channels, including NMDARs, in different conditions. This technique was able to discriminate differential Ca²⁺/Na⁺ permeation ratio through different receptor channels, and through the same channel in different conditions. With this method, we confirmed that EU1794-4, a

SUBMITTER: D'Andrea T 

PROVIDER: S-EPMC11249768 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature