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New Insights in <i>bla</i><sub>KPC</sub> Gene Mobilization in <i>Pseudomonas aeruginosa</i>: Acquisition of <i>bla</i><sub>KPC-3</sub> and Identification of a New Tn<i>2</i>-like NTE Mobilizing <i>bla</i><sub>KPC-2</sub>.

ABSTRACT: Carbapenem-resistant Pseudomonas aeruginosa is a major cause of healthcare associated infections in hospitalized patients and what is more warring with reduced therapeutic options. The KPC is a powerful enzyme capable of hydrolyzing the carbapenems, described first in Klebsiella pneumoniae and it already has found in P. aeruginosa.Objective: To perform a comparative genomic analysis of two new genetic platforms mobilizing the blaKPC-2 and blaKPC-3 in two ST111 and ST235 pandemic clones of P. aeruginosa in Colombia, South America. Methods: Sixty-six blaKPC-harboring P. aeruginosa isolates were identified and characterized during a prospective study conducted in six high complex hospitals in Colombia. Genetic platforms mobilizing the blaKPC were analyzed. Results: The blaKPC-2 and blaKPC-3 were identified in 24 and 42 isolates, respectively. The blaKPC-2-harboring isolates belonged to ST235 and blaKPC-3 to ST111. The whole genome sequencing indicated that the blaKPC-3 gene was mobilized by the Tn4401b within a 55-kb-size environmental origin plasmid, which, in other isolates, was inserted into the chromosome through a transposition event of ISPa38. Regarding the blaKPC-2 gene, this was mobilized by a new Non-Tn4401 Element (NTE) derived from transposon Tn2 (proposed as variant IIg), which has been transposed into a 43-Kb-size little-studied plasmid related to Klebsiella spp. Conclusions: Our results reveal a new acquisition event of blaKPC in P. aeruginosa, in this case blaKPC-3. Likewise, the pandemic high-risk clones ST111 and ST235 of P. aeruginosa continues to spread blaKPC gene through different mobile genetic elements, jumping of conventional Tn4401b and acquiring new Tn2-derived NTE, which were inserted in diverse plasmids.

SUBMITTER: Abril D 

PROVIDER: S-EPMC12466754 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature

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New Insights in &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;KPC&lt;/sub&gt; Gene Mobilization in &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt;: Acquisition of &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;KPC-3&lt;/sub&gt; and Identification of a New Tn&lt;i&gt;2&lt;/i&gt;-like NTE Mobilizing &lt;i&gt;bla&lt;/i&gt;&lt;sub&gt;KPC-2&lt;/sub&gt;.

Abril Deisy D   Bravo-Ojeda Juan J   Garcia Julio-Cesar JC   Leal-Castro Aura Lucia AL   Saavedra-Trujillo Carlos Humberto CH   Madroñero Johana J   Bustos Rosa-Helena RH   Marquez-Ortiz Ricaurte Alejandro RA   Rozo Zayda Lorena Corredor ZLC   Gómez Natasha Vanegas NV   Escobar-Pérez Javier J  

Antibiotics (Basel, Switzerland) 20250919 9

Carbapenem-resistant <i>Pseudomonas aeruginosa</i> is a major cause of healthcare associated infections in hospitalized patients and what is more warring with reduced therapeutic options. The KPC is a powerful enzyme capable of hydrolyzing the carbapenems, described first in <i>Klebsiella pneumoniae</i> and it already has found in <i>P. aeruginosa.</i><b>Objective</b>: To perform a comparative genomic analysis of two new genetic platforms mobilizing the <i>bla</i><sub>KPC-2</sub> and <i>bla</i><  ...[more]

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