Detection of metabolic change in glioblastoma cells after radiotherapy using hyperpolarized ¹³ C-MRI.

ABSTRACT: Dynamic nuclear polarization (DNP) of ¹³ C-labeled substrates enables the use of magnetic resonance imaging (MRI) to monitor specific enzymatic reactions in tumors and offers an opportunity to investigate these differences. In this study, DNP-MRI chemical shift imaging with hyperpolarized [1-¹³ C] pyruvate was conducted to evaluate the metabolic change in glycolytic profiles after radiation of two glioma stem-like cell-derived gliomas (GBMJ1 and NSC11) and an adherent human glioblastoma cell line (U251) in an orthotopic xenograft mouse model. The DNP-MRI showed an increase in Lac/Pyr at 6 and 16 h after irradiation (18% ± 4% and 14% ± 3%, respectively; mean ± SEM) compared with unirradiated controls in GBMJ1 tumors, whereas no significant change was observed in U251 a

SUBMITTER: Kawai T 

PROVIDER: S-EPMC8243917 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature