Performance of the Abbott Architect Immuno-Chemiluminometric NT-proBNP Assay.

ABSTRACT: Background: We evaluated the performance of the Abbott N-terminal pro-brain natriuretic peptide (NT-proBNP) assay against the Roche NT-proBNP immunoassay across two sites. Methods: Precision, linearity, and sensitivity studies were performed. A combined method of comparison and regression analysis was performed between the Roche and Abbott assays using samples from both sites (n = 494). To verify biotin interference, lyophilised biotin powder was reconstituted and spiked into serum samples at two medical decision levels (final concentration 500/4250 ng/mL) and compared to controls. NT-proBNP was also measured in anonymised leftover sera (n = 388) in a cardio-renal healthy population and stratified into three age bands-<50 (n = 145), 50-75 (n = 183) and >75 (n = 60).

SUBMITTER: Lau CS 

PROVIDER: S-EPMC9140882 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature