Dataset Information
IgE+ plasmablasts predict the onset of clinical allergy.
Ontology highlight
ABSTRACT: Introduction
IgE+ plasmablasts develop following allergen exposure and B cell activation. They secrete IgE and therefore are directly linked to maintain the mechanisms of IgE-mediated allergies. Here, we show that the presence of IgE+ plasmablasts in peripheral blood not only coincides with clinical allergy, but also predicts the upcoming development of clinical disease.Methods
Using an equine model of naturally occurring allergy, we compared the timing of allergen exposure, arrival of IgE+ plasmablasts in peripheral blood, and onset of clinical disease.Results
We found that IgE+ plasmablasts predict the development of clinical allergy by at least 3 weeks and can be measured directly by flow cytometry or by IgE secretion following in vitro culture. We also compared the IgE secretion by IgE+ plasmablasts with total plasma IgE concentrations and found that while IgE secretion consistently correlates with clinical allergy, total plasma IgE does not.Discussion
Together, we describe IgE+ plasmablasts as a reliable and sensitive predictive biomarker of allergic disease development.
SUBMITTER: Simonin EM
PROVIDER: S-EPMC9932261 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Publications
IgE+ plasmablasts predict the onset of clinical allergy.
Frontiers in immunology 20230202
<h4>Introduction</h4>IgE+ plasmablasts develop following allergen exposure and B cell activation. They secrete IgE and therefore are directly linked to maintain the mechanisms of IgE-mediated allergies. Here, we show that the presence of IgE+ plasmablasts in peripheral blood not only coincides with clinical allergy, but also predicts the upcoming development of clinical disease.<h4>Methods</h4>Using an equine model of naturally occurring allergy, we compared the timing of allergen exposure, arri ...[more]