Proteomics

Dataset Information

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LC-MS/MS identification of RPL22 interaction proteins

ABSTRACT: Proteins obtained in Co-IP were subjected to SDS-PAGE gel electrophoresis and Coomassie brilliant blue staining. After decolorization, the SDS-PAGE gel was sent to the mass spectrometry platform of the Institute of Biophysics, Chinese Academy of Sciences for subsequent processing and mass spectrometry detection.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaoyu Jiang  

PROVIDER: PXD044052 | iProX | Mon Jul 24 00:00:00 GMT+01:00 2023

REPOSITORIES: iProX

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Publications

CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver.

Li Hong-Yu HY   Wang Min M   Jiang Xiaoyu X   Jing Yaobin Y   Wu Zeming Z   He Yifang Y   Yan Kaowen K   Sun Shuhui S   Ma Shuai S   Ji Zhejun Z   Wang Si S   Belmonte Juan Carlos Izpisua JCI   Qu Jing J   Zhang Weiqi W   Wei Taotao T   Liu Guang-Hui GH  

Nucleic acids research 20241001 19

Dysfunction of the ribosome manifests during cellular senescence and contributes to tissue aging, functional decline, and development of aging-related disorders in ways that have remained enigmatic. Here, we conducted a comprehensive CRISPR-based loss-of-function (LOF) screen of ribosome-associated genes (RAGs) in human mesenchymal progenitor cells (hMPCs). Through this approach, we identified ribosomal protein L22 (RPL22) as the foremost RAG whose deficiency mitigates the effects of cellular se  ...[more]

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