Sort   by:  
 Page size 
UnknownModeling contaminants in AP-MS/MS experiments.
Not available
S-EPMC4494835 | biostudies-literature
ProteomicsNicotiana benthamiana AP-MS for TSWV NSs
Despite the advancement of structural and functional research on TSWV NSs, its protein-interacting targets in host plants are still largely unknown. In this research, we investigated the NSs-interacting proteins in Nicotiana benthamiana via affinity purification and mass spectrometry (AP-MS) analysi...
ORGANISM(S): Nicotiana benthamiana 
2021-01-04 | PXD022401 | Pride
Tswv nss Ap-ms Nicotiana benthamiana 
ProteomicsMapping ISG15 sites on GAPDH and PGK1 by AP-MS
We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD071724 | Pride
Ap-ms Pgk1 Gapdh Isg15 Glycolysis 
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs usi...
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD012800 | Pride
Interaction proteomics Low input Ap-ms 
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-10 | PXD011323 | Pride
Interaction proteomics Low input Ap-ms 
ProteomicsSMARCD1 Affinity Purification-mass Spectrometry (AP-MS) in HEK-293 Cells
This study reports the identification of the FLAG-tagged SMARCD1 protein interactome in HEK-293 cells using Affinity Purification-Mass Spectrometry (AP-MS). The data provides a comprehensive list of proteins that physically associate with SMARCD1, thereby contributing to the understanding of its mol...
ORGANISM(S): Homo sapiens (Human) 
2026-07-09 | PXD068854 | Pride
Ap-ms Smarcd1 
ProteomicsParadoxical non-catalytic kinase functions are driven by inhibitor-induced displacement of autoinhibitory domains
Protein kinases are crucial regulators of cellular processes, and their dysregulation is frequently associated with human disorders. ATP-competitive type I inhibitors are widely used to block aberrant kinase activity. However, their effect on non-catalytic kinase functions such as conformation and p...
ORGANISM(S): Homo sapiens (Human) 
2026-06-17 | PXD069830 | Pride
Lip-ms Proximity labeling Structural proteomics Ap-ms Bioid 
ProteomicsIdentify the interacting proteins of FBXL4 by AP-MS
Affinity purification coupled with mass spectrometry (AP-MS) is powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex.Mass spectrometry was performed to identify the interacting proteins of FBXL4 fro...
ORGANISM(S): Homo sapiens (Human) 
2024-05-12 | PXD040601 | Pride
Human Fbxl4 Ap-ms 
TranscriptomicsJanus-Like Opposing Roles of CD47 in Autoimmune Brain Inflammation in Human and Mice
Gene transcripts and proteins expressed during disease pathogenesis identify targets for therapy. We performed microarray analysis of histologically characterized multiple sclerosis (MS) brain lesions in comparison with control brain samples to identify differentially expressed molecules. We identif...
ORGANISM(S): Homo sapiens 
2012-06-23 | E-GEOD-38010 | biostudies-arrayexpress
ProteomicsFBXL6 interactome analysis via AP-MS
Interactome analysis via AP-MS identify CatG (Cathepsin G) as a FBXL6 interactor.
ORGANISM(S): Homo sapiens (Human) 
2024-07-15 | PXD051269 | Pride
Ap-ms E3 ubiquitin ligase Interactome Aml 
Sort   by:  
 Page size