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MetabolomicsCell type-specific proteomics uncovers a RAF15-SnRK2.6/OST1 kinase cascade in guard cells

Multicellular organisms such as plants contain various cell types with specialized functions. Analyzing the characteristics of each cell type reveals specific cell functions and enhances our understanding of organization and function at the organismal level. Guard cells (GCs) are specialized epid...

2024-09-03 | MTBLS3997 | MetaboLights
ProteomicsProteomics characterization of extracellular vesicles sorted by flow cytometry reveals a disease-specific molecular cross-talk from cerebrospinal fluid and tears in multiple sclerosis
Several proteomics studies have been conducted to identify new cerebrospinal fluid (CSF) biomarkers in Multiple Sclerosis (MuS). However, the complexity of CSF and its invasive collection, limits its use. Therefore, the goal of biomarker research in MuS is to identify novel distinctive targets in CS...
ORGANISM(S): Homo sapiens (Human) 
2019-06-06 | PXD013794 | Pride
Proteomics  extracellular vesicles  facs sorting  csf  tears  multiple sclerosis. 
ProteomicsAnalysis of barley chromosomal proteome: a multiapproach exploration of flow cytometry-sorted mitotic chromosomes
Proteins contribute to the structure of chromatin and regulate its functions. The basic building block of chromatin in eukaryotes is the nucleosome containing histones. Structural maintenance of chromosomes complexes perform the assembly of genomic DNA into the higher organizational structures of in...
ORGANISM(S): Hordeum vulgare (Barley) 
2021-07-21 | PXD024689 | Pride
Prediction Proteomics Mass spectrometry Flow cytometry Barley Chromatin Chromosome 
ProteomicsProteomic characterization of sorted peroxisomes
Proteomic analysis of sorted peroxysomes (old, young, and middle-aged).
ORGANISM(S): Homo sapiens (Human) 
2025-03-04 | PXD028679 | Pride
Proteomics Peroxisome 
ProteomicsProteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations
The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with ...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2017-11-02 | PXD005812 | Pride
Corynebacterium glutamicum Proteome analysis Facs Label-free quantification Cellular heterogeneity Bcaa producer 
ProteomicsTMT-proteomics of sorted MC38+ tumor cells with high-fat diet
Obesity is a major cancer risk factor, but the underlying molecular mechanisms are not always known. In this study, we look at proteome remodeling in cancer cells with obesity, comparing tumor cells sorted from mice fed high-fat versus control diet. We conducted 10-plex TMT-proteomics on GFP+ MC38 c...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-16 | PXD019495 | Pride
Obesity Mouse Biological Colorectal cancer 
ProteomicsFlow Cytometry Analysis of Circulating Extracellular Vesicle Subtypes from Fresh Body Fluids
Extracellular vesicles (EVs) are released by shedding during different physiological processes and are increasingly thought to be new potential biomarkers. However, the impact of pre-analytical processing phases on the final measurement is not predictable and, for this reason, the translation of bas...
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD022807 | Pride
Extracellular vesicles  polychromatic flow cytometry  biomarkers  proteomics  fresh peripheral blood. 
ProteomicsGlobal proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons
The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTom...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-15 | PXD031535 | Pride
Atg5 ko In-vivo proteome Neurons Autophagy 
ProteomicsMicroproteomics application for the limited number of cells
Mass spectrometry (MS)-based proteomics explores in-depth proteome, however, the analysis of cell subsets, which might be minor population, is hampered due to a multistep procedure for sample preparation and purification. Along those lines, optimization of proteomics workflow toward limited number...
ORGANISM(S): Homo Sapiens (human) 
2017-07-18 | PXD005096 | JPOST Repository
UnknownUse of fluorescence-activated vesicle sorting for isolation of Naked2-associated, basolaterally targeted exocytic vesicles for proteomics analysis.
Not available
S-EPMC2528074 | biostudies-literature
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