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ProteomicsIdentifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Mass spectrometry‐based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically‐altered enzymecofactor systems. We describe a method that combines stable γ‐[18O2]‐ATP with classical in v...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD002133 | Pride
Human Kinase substrates  in vitro kinase assay  proteomics  mass spectrometry  heavy atp  non receptor tyrosine kinase abl1 
TranscriptomicsHeavy metal resistance in Cupriavidus metallidurans CH34 is governed by an intricate transcriptional network.
Background: The high number of heavy metal resistance genes in the soil bacterium Cupriavidus metallidurans CH34 makes it an interesting model organism to study microbial responses to heavy metals. Results: In this study the transcriptional response of this bacterium was measured after challenging i...
ORGANISM(S): Cupriavidus metallidurans CH34 
2011-06-28 | E-GEOD-23876 | biostudies-arrayexpress
MetabolomicsPb Stress and Ectomycorrhizas: Strong Protective Proteomic Responses in Poplar Roots Inoculated with Paxillus involutus Isolate and Characterized by Low Root Colonization Intensity
The commonly observed increased heavy metal tolerance of ectomycorrhized plants is usually linked with the protective role of the fungal hyphae covering colonized plant root tips. However, the molecular tolerance mechanisms in heavy metal stressed low-colonized ectormyocrrhizal plants characterized ...
2022-01-11 | MTBLS1847 | MetaboLights
ProteomicsHuman THP1 macrophages after interacting with C. albicans, using ATP-binding protein enrichment strategy
Macrophages are involved in the primary human host response to pathogens such as Candida albicans. After pathogen recognition by specific receptors, a cascade of signaling pathways is activated, leading to the production of cytokines, chemokines and antimicrobial peptides. ATP binding proteins are c...
ORGANISM(S): Candida albicans (Yeast) 
2019-04-18 | PXD009938 | Pride
Phosphopeptides Silac Atp-binding proteins Macrophages Candida albicans 
ProteomicsHuman AHH1 cells heavy ion irradiation LC-MSMS
Heavy ion radiotherapy has unfolded a great promise for cancer therapy. Understanding the cellular response mechanism to heavy ion radiation is required for exploring the measures of overcoming the devastating side effects. Here, we performed a quantitative proteomics to investigate the mechanis...
ORGANISM(S): Homo sapiens (Human) 
2018-11-07 | PXD008351 | Pride
Human Lc-msms Heavy ion radiation Ahh1 cells 
TranscriptomicsA long noncoding RNA protects the heart from pathological hypertrophy
The role of long noncoding RNA (lncRNA) in adult hearts is unknown; also unclear is how lncRNA modulates nucleosome remodelling. An estimated 70% of mouse genes undergo antisense transcription1, including myosin heavy chain 7 (Myh7), which encodes molecular motor proteins for heart contraction2. Her...
ORGANISM(S): Homo sapiens 
2014-08-05 | E-GEOD-49716 | biostudies-arrayexpress
ProteomicsThe Function of Ectopic ATP Synthase in Extracellular Vesicles.
Ectopic adenosine triphosphate synthase (eATP synthase) was found on the cell surface to generate extracellular ATP in various cancer cells. Recently using proteomics approach, several reports identified ATP synthase in extracellular vesicles (EVs) which are important in cell-cell communications. Ho...
ORGANISM(S): Homo sapiens (Human) 
2023-05-22 | PXD014995 | Pride
Ectopic atp synthases  extracellular vesicles  proteomic analysis  serum depletion  human lymphoma jurkat t cells 
ProteomicsThe mitochondrial Hsp70 controls the assembly of the F1FO-ATP synthase
The mitochondrial F1FO-ATP synthase produces the bulk of cellular ATP. The soluble F1 domain contains the catalytic head that is linked via the central stalk and the peripheral stalk to the membrane embedded rotor of the Fo domain. The assembly of the F1 domain and its linkage to the peripheral stal...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-11 | PXD033024 | Pride
Mitochondria F1fo-atp synthase Pulldown Mthsp70 
TranscriptomicsTranscriptomic and metabolic responses of Staphylococcus aureus exposed to supra-physiological temperatures
Previous evaluation by different molecular and physiological assays of Staphylococcus aureus (S. aureus) responses to heat shock exposure yielded a still fragmentary view of the mechanisms determining bacterial survival or death at supra-physiological temperatures. This study analyzed diverse facets...
ORGANISM(S): Staphylococcus aureus 
2010-06-19 | E-GEOD-12920 | biostudies-arrayexpress
ProteomicsCharacterisation of a highly diverged putative mitochondrial ATP synthase peripheral stalk subunit b in Trypanosoma brucei
The mitochondrial F1Fo ATP synthase of Trypanosoma brucei has been studied in detail. Whereas its F1 moiety is relatively highly conserved in structure and composition, the same is not the case for the Fo part and the peripheral stalk. A core subunit of the latter, the normally conserved subunit b, ...
ORGANISM(S): Trypanosoma brucei 
2022-05-20 | PXD031259 | Pride
Silac Trypanosoma brucei Quantitative proteomics Rnai 
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