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ProteomicsProteomic analysis links truncated tau to lysosome motility, autophagy and endo-lysosomal dysfunction
Tauopathies are characterized by the progressive accumulation of abnormal tau species, which disrupt the autophagy-lysosomal pathway (ALP), a critical system for degrading intracellular macromolecules and aggregated proteins, causing toxicity and cell death. This study investigates the impact of the...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-22 | PXD062128 | Pride
Tauopathies Toxicity Proteomics Mass spectrometry Autosomal 
ProteomicsSystematic comparison of strategies for the enrichment of lysosomes by data independent acquisition
We describe a comparison of individual approaches for the enrichment of lysosomes comparing four different strategies using data independent acquisition (DIA). As a benchmark, we chose a crude enrichment strategy, which is the centrifugation of the PNS at 20,000 x g termed organelle enriched pellet ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-21 | PXD015098 | Pride
Enrichment methods Lysosomes Data independent acquisition (dia) 
UnknownBenchmarking Lysosome Enrichment Methods: A Guide for Research and Clinical Translation.
Not available
S-EPMC12921670 | biostudies-literature
UnknownMorpholine-Facilitated Enrichment-Triggered Delivery of Carbon Monoxide to Lysosome: A Feasibility Study.
Not available
S-EPMC12534755 | biostudies-literature
ProteomicsTwo step enrichment facilitates background reduction for proteomic analysis of lysosomes
Lysosomes comprise the main degradative compartment of almost all mammalian cells and are involved in various cellular functions, most of which are catalyzed by the lysosomal proteome. Lysosomal proteins are low abundant, complicating their analysis by mass spectrometry-based proteomics. To increase...
ORGANISM(S): Homo sapiens (Human) 
2024-08-01 | PXD048696 | Pride
Lysosomes  lysosome enrichment  superparamagnetic iron oxide particles (spions)  tmem192 Mass spectrometry  proteomics  organelles Hek 293 
ProteomicsProteome profiling of enriched lysosomes obtained from four commercial isolation methodologies.
Lysosomes are membrane-bound cell organelles that can be found in all mammalian cells. They contain digestive enzymes and are involved in multiple processes, including degradation of worn-out cell parts, antigen presentation and induction of programmed cell death. Mass spectrometry studies of lysoso...
ORGANISM(S): Homo sapiens (Human) 
2026-02-23 | PXD040003 | Pride
Tmt Lysosome Sp3 Thp-1 Organelle enrichment 
GenomicsLoss of Gak leads to lysosome dysfunction and immune modulation in podocytes
Loss of Gak leads to lysosome dysfunction and immune modulation in podocytes
PRJNA1288537 | ENA
TranscriptomicsLoss of Gak leads to lysosome dysfunction and immune modulation in podocytes
Lysosome impairment causes cellular and organ dysfunction, highlighting its crucial role in homeostasis. In this study, we uncovered the indispensable role of cyclin G-associated kinase (GAK) in maintaining podocyte lysosome integrity. We previously showed that podocyte-specific loss of Gak (Gak-KO)...
ORGANISM(S): Mus musculus 
2026-06-18 | GSE302106 | GEO
ProteomicsMulti cell line comparison of lysosome enriched fractions reveals cell type specific features and novel lysosomal proteins
Lysosomes are the main degradative organelles of mammalian cells and are responsible for the breakdown of the majority of cellular macromolecules and the recycling of their building blocks. To accomplish this task, lysosomes contain ~60 hydrolases. Malfunction of these proteins, as well as other pro...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-02-21 | PXD020600 | Pride
Isolated lysosomes Dia Human Silac Lfq Mouse Ibaq Lysosome Cell line comparison 
UnknownRab7: a key to lysosome biogenesis.
Not available
S-EPMC14786 | biostudies-literature
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