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UnknownChip-based nLC-TOF-MS is a highly stable technology for large-scale high-throughput analyses.
Not available
S-EPMC3640767 | biostudies-literature
ProteomicsAGP nLC-MS/MS and nLC-FAIMS-MS/MS
Bottom-up nLC-MS/MS glycoprotein mass spectrometry workflows rely on the generation of a mixture of unmodified and glycosylated peptides via proteolysis. Such methods are challenged by suppression of hydrophilic glycopeptide ions by more abundant, hydrophobic, and readily ionizable unmodified peptid...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036667 | Pride
N-glycopeptide High-field asymmetric waveform ion mobility spectrometry Glycoproteomics Agp Alpha-1-acid glycoprotein Faims 
UnknownTwo-Dimensional-PAGE Coupled with nLC-MS/MS-Based Identification of Differentially Expressed Proteins and Tumorigenic Pathways in MCF7 Breast Cancer Cells Transfected for JTB Protein Silencing.
Not available
S-EPMC10673289 | biostudies-literature
ProteomicsHuman Serum Immunoglobulin N-Glycosylation using nLC-MS/MS
Here we employ a nLC-MS/MS method to separate small amounts of purified immunoglobulins to characterize the N-glycan reportoire and site occupancy of bulk serum antibodies. Using this method, we have established, for the first time within individual donors, the N-linked glycan repotoire for bulk IgG...
ORGANISM(S): Homo sapiens (Human) 
2019-01-24 | PXD010911 | Pride
Human Igm Serum N-glycosylation Iga2 Iga1 Glycopeptides Igg1 Igg4 Immunoglobulins 
MetabolomicsUncovering the Complexity of the Yeast Lipidome by Means of nLC/NSI-MS/MS
Saccharomyces cerevisiae is a eukaryotic model organism widely used for the investigation of fundamental cellular processes and disease mechanisms. Consequently, the lipid landscape of yeast has been extensively investigated and up to this day the lipidome is considered as rather basic. Here, we use...
2021-01-08 | MTBLS2119 | MetaboLights
Proteomics2D-PAGE coupled with nLC-MS/MS-based identification of differentialy expressed proteins and tumorigenic pathways in MCF7 breast cancer cell line transfected for JTB protein silencing
Identification of new cancer-associated genes/proteins, characterization of their expression vari-ation, the interactomics-based assessment of differentially expressed genes/proteins (DEGs/DEPs), and understanding the tumorigenic pathways and biological processes involved in BC genesis and progressi...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD046265 | Pride
Breast cancer (bc)  mcf7  jtb protein silencing  overexpressed jtb-interactome  downregulated jtb-interactome  tumorigenic pathways 
ProteomicsSphingobium indicum B90A nLC-MS/MS
The project aims to to understand the response of the lin genes in Sphingobium indicum B90A under the stress of HCH isomers and the metabolites formed during degradation of hexachlorocyclohexane (HCH). Entire cell proteome from Sphingobium indicum B90A was extracted in presence of four HCH isomers. ...
ORGANISM(S): Sphingobium indicum B90A 
2021-04-06 | PXD018599 | Pride
Sphingobium indicum b90a Hexacholorocyclohexane Stress response Nlc-ms/ms Proteome 
ProteomicsAnalysis of EYA3 phosphorylation by Src kinase
Data-dependent nLC-MS/MS analysis of EYA3 phosphorylation by Src protein tyrosine kinase
ORGANISM(S): Homo sapiens (Human) 
2019-12-17 | PXD016518 | Pride
Tyrosine phosphorylation Eya3 Nlc-ms/ms Src 
ProteomicsAnalysis of EYA3 phosphorylation by Src kinase
Data-dependent nLC-MS/MS analysis of EYA3 phosphorylation by Src protein tyrosine kinase
ORGANISM(S): Homo sapiens (Human) 
2019-12-17 | PXD016520 | Pride
Tyrosine phosphorylation Eya3 Nlc-ms/ms Src 
ProteomicsAutomated identification of the Cowpox virus (Brighton Red) strain in HEp-2 cells using nLC-MS/MS spectra
Accompanying benchmarking sample for "TaxIt: An iterative computational pipeline for untargeted strain-level identification using MS/MS spectra from pathogenic single-organism samples": Untargeted accurate strain-level classification of a priori unidentified organisms using tandem mass spectrometry...
ORGANISM(S): Homo sapiens (Human) Cowpox virus (Brighton Red) 
2020-05-11 | PXD014913 | Pride
Cowpox virus (brighton red) Human Nlc-ms/ms 
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