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ProteomicsPEPPI-MS- and GELFrEE-fractionated E. coli MG1655 whole cell lysate
Supplemental Files accompanying manuscript “PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry”.
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2020-04-08 | MSV000085258 | MassIVE
ProteomicsPassive elution of proteoforms from polyacrylamide gels combined with anion exchange purification for in-depth top-down analysis of the human low molecular weight proteome.
We developed a novel sample preparation method by combining Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS (PEPPI-MS) fractionation with Anion-Exchange disc-assisted Sequential sample Preparation (AnExSP) purification, and evaluated its performance in TDP analysis by co...
ORGANISM(S): Homo sapiens (Human) 
2022-09-26 | PXD034353 | Pride
Sds-page Top-down Peppi Anion exchange Stagetip 
UnknownPEPPI: Whole-proteome Protein-protein Interaction Prediction through Structure and Sequence Similarity, Functional Association, and Machine Learning.
Not available
S-EPMC8897833 | biostudies-literature
UnknownPEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry.
Not available
S-EPMC8141340 | biostudies-literature
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
Precise prefractionation of proteome samples is a potent method for realizing in depth analysis in top-down proteomics. PEPPI MS (Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS), a gel-based sample fractionation method, enables high-resolution proteome fractionation bas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000095992 | MassIVE
ProteomicsIntegrated Protein Precipitation–SPE Workflow for Deep Top-Down Proteomic Analysis of Human Serum
Top-down proteomics of human serum is hindered by extreme dynamic range and cumbersome prefractionation. Here we establish a fully solution-based protein precipitation–solid-phase extraction (PP–SPE) workflow for deep, low-molecular-weight-biased proteoform profiling. Using systematically optimized ...
ORGANISM(S): Homo Sapiens 
PXD073228 |
UnknownOutcomes of a Mobile App to Monitor Patient-Reported Outcomes in Rheumatoid Arthritis: A Randomized Controlled Trial.
Not available
S-EPMC8330418 | biostudies-literature
UnknownEffects of a Proactive Interdisciplinary Self-Management Program on Patient Self-Efficacy and Participation During Practice Nurse Consultations: A Randomized Controlled Trial in Type 2 Diabetes.
Not available
S-EPMC7011933 | biostudies-literature
ProteomicsGeLC-FAIMS-MS Workflow for In-Depth Middle-Down Proteomics
Middle-down proteomics is an analytical approach whereby protein samples are digested with proteases such as Glu-C to generate large peptides (>3 kDa) that are then analyzed by mass spectrometry. This method is useful for characterizing high-molecular-weight proteins that are difficult to detect by ...
ORGANISM(S): Homo sapiens (Human) 
2024-11-20 | PXD041358 | Pride
Glu-c digestion Proteoform Middle-down proteomics Lc-faims orbitrap mass spectrometry Peppi-ms Anexsp 
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