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ProteomicsEvaluation of the technical reproducibility of serum proteome sample preparation methods to support clinical proteomics applications
Evaluation of the efficiency and reproducibility of sample prepration methods using iST-BCT kit from PreOmics or SMART Trypsin Kit (Thermo Fisher) with in-house Rapigest protocol in human serum samples prior to mass spectrometry analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-04-07 | PXD051002 | Pride
Serum  proteomics  biomarker  mass spectrometry  rapigest sf  ist-bct  smart digest trypsin 
ProteomicsCFAP43 interactome - A SF-TAP tagged CFAP43 fusion protein was expressed in HEK293T cells. Protein complexes were purified by tandem affinity purification to identify the CFAP43-associated protein complex.
A SF-TAP tagged CFAP43 fusion protein was expressed in HEK293T cells. Protein complexes were purified by tandem affinity purification to identify the CFAP43-associated protein complex.
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2019-04-11 | PXD009786 | Pride
Human Mouse Protein complex 
ProteomicsMetabolomic and Proteomic Stratification of Equine Osteoarthritis
Osteoarthritis (OA) is characterised by loss of articular cartilage, synovial membrane dysfunction and subchondral sclerosis. Few studies have used a global approach to stratify equine synovial fluid (SF) molecular profiles according to OA severity. SF was collected from 58 metacarpophalangeal (MCP)...
ORGANISM(S): Equus caballus (Horse) 
2025-02-17 | PXD019842 | Pride
Mass spectrometry Equine Synovial fluid Proteomics Osteoarthritis 
ProteomicsOptimisation of Synovial Fluid Collection and Processing for NMR Metabolomics and LC-MS/MS Proteomics
Synovial fluid (SF) is of great interest for the investigation of orthopaedic pathologies as it is in close proximity to various tissues which are primarily altered during these disease processes and can be collected using minimally invasive protocols. Therefore, SF has substantial potential for imp...
ORGANISM(S): Equus caballus (Horse) 
2020-04-06 | PXD017069 | Pride
Metabolomics Mass spectrometry Synovial fluid Nuclear magnetic resonance Proteomics Lys-c endopeptidase 
ProteomicsQuantitation of plasma protein concentrations by mass spectrometry without the use of isotopic standards. C4PR_LIV
We applied a non targeted mass spectrometric assay (LCMSE), to quantify 55 abundant plasma proteins in specimens from 31 healthy volunteers. Quantitation was done by HI3 peptide measurement using a single internal standard to estimate protein concentrations. Results for 7 apolipoproteins were compar...
ORGANISM(S): Homo sapiens (Human) 
2015-10-20 | PXD000347 | Pride
Lcmse Quantitation Mass spectrometry Plasma proteins Proteomics Immunoassay 
ProteomicsEquine synovial fluid
The aim of the study was to characterise the protein complement of synovial fluid (SF) in health and osteoarthritis (OA) using liquid chromatography mass spectrometry (LC-MS/MS) following peptide-based depletion of high abundance proteins. SF was used from nine normal and nine OA Thoroughbred hors...
ORGANISM(S): Equus caballus (Horse) 
2022-03-02 | PXD001509 | Pride
Osteoarthritis Synovial fluid Equine 
ProteomicsStructure of the bc1-cbb3 respiratory supercomplex from Pseudomonas aeruginosa
The sample was digested using MS-grade trypsin/Lys-C (Promega) to a final ratio of 1:20 (w/w)(SC: protease) using RapiGest SF surfactant (Waters) according to manufacturers instructions. Tryptic peptides were then separated on a 60-minute H2O: acetonitrile (ACN) gradient using a Waters ACQUITY I-Cla...
ORGANISM(S): Pseudomonas Aeruginosa Pao1 (ncbitaxon:208964) 
2023-04-27 | MSV000091814 | MassIVE
ProteomicsThe Synovial Fluid Proteome Differentiates between Septic and Nonseptic Articular Pathologies
Presently few studies have investigated the proteomic profile of equine synovial fluid (SF) using high mass resolution mass spectrometer and in-solution trypsin digestion and to our knowledge none have investigated protein profile changes septic SF in any species. In this study we have used mass spe...
ORGANISM(S): Equus caballus (Horse) 
2019-05-01 | PXD011276 | Pride
Synovial fluid Proteomics Sepsis 
ProteomicsNanoTPOT: Enhanced Sample Preparation for Quantitative Na-noproteomic Analysis (revision work)
With the ever-growing need for protein-level understanding in pathological research, proteomics researchers thrive to exam-ine detailed proteome dynamics using crucial, yet often limited, primary and clinical samples. Aside from mass spectrometer instrumentation advancement, a single-tube-based high...
ORGANISM(S): Homo sapiens (Human) 
2021-03-16 | PXD018247 | Pride
Hela Tmt 
UnknownEvaluation of Serum Proteome Sample Preparation Methods to Support Clinical Proteomics Applications.
Not available
S-EPMC11546599 | biostudies-literature
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