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MetabolomicsTear lipidomics profiling reveals inflammatory dry eye disease-associated lipid metabolic remodeling at the ocular surface
This study aimed to investigate whether inflammatory dry eye disease (DED) is accompanied by abnormal lipid metabolic remodeling at the ocular surface. Tear samples were collected from patients with DED and matched healthy controls. Lipidomics profiling was performed using LC–MS/MS with a standardiz...
2026-04-03 | MTBLS14218 | MetaboLights
MetabolomicsTear lipidomics profiling reveals ocular-surface lipid remodeling associated with organelle stress in a mouse model of inflammatory dry eye disease
This study aimed to determine whether dry eye disease (DED) is accompanied by ocular-surface lipid remodeling and whether such changes converge on organelle stress–associated pathways in a mouse model of inflammatory dry eye. Tear samples were collected from mice and analyzed by LC–MS/MS–based non-t...
2026-04-04 | MTBLS14217 | MetaboLights
ProteomicsPrecision in Tear Fluid Biomarker Discovery: Quantitative Proteomic Profiling of Small-Volume, Individual Samples Using Capillary Tube Collection
Tear fluid, a complex biofluid that contains thousands of proteins and can be collected non-invasively, has emerged as a promising source of biomarkers for ocular and systemic health. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is currently the primary method for discovering novel biom...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD060101 | Pride
1. tear proteomics  2. tear biomarker  3. biomarker discovery  4. capillary collection  5. lc-ms/ms 
ProteomicsInvestigation of inter- and intra-day variability of tear fluid regarding flow rate, protein concentration as well as protein composition
Purpose: Determination of inter- and intra-day variations in tear flow rate, tear fluid protein concentration as well as protein composition regarding their impact for future biomarker studies.Methods: Tear fluid was collected non-invasively from 18 healthy subjects performing Schirmer tests at four...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD037811 | Pride
Mass spectrometry Tear fluid Schirmer test Tear flow rate  tear volume Protein concentration 
ProteomicsRapid Tear Proteomics via In-Capillary Digestion for Biomarker Discovery
Tear fluid is emerging as a valuable resource for biomarker discovery, but its low sample volume and dynamic composition pose significant challenges for analytical testing. Most tear proteomics studies have focused on samples collected with Schirmer strips, while capillary tube collection has receiv...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD056978 | Pride
1. tear proteomics  2. tear biomarker  3. biomarker discovery  4. point-of-care testing  5. poct  6. lc-ms/ms 
ProteomicsComparison of Different Mass-Spectrometry Workflows for the Proteomic Analysis of Tear Fluid
Proteomic analysis of tear fluid is challenging mainly due to a wide dynamic range of proteins and small sample volume. However, recent advancements in mass spectrometry have revolutionized the field of proteomics through its unprecedented depth, speed, and accuracy even with small sample volumes. I...
ORGANISM(S): Homo sapiens (Human) 
2022-04-04 | PXD030990 | Pride
Tear film  proteomics  biomarkers  dry eye disease  ocular surface  mass spectrometry 
ProteomicsEye opening discoveries: uncovering lacritin glycoforms and the tear fluid glycoproteome
Tear fluid comprises a diverse group of extracellular glycoproteins which are critical for ocular homeostasis. Within the tear fluid glycoproteome, lacritin is highly expressed and plays a key role in immune response, tear secretion, and antimicrobial activity. Importantly, glycosylation constitutes...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD064788 | Pride
Glycoproteomics Mucin Mucinase Lacritin Mass spectrometry Immunoglobulin a Tear fluid 
ProteomicsComparative analysis of tear proteomics in PAX6 mutant and control mice of both sexes before and after abrasion
Our team is working on aniridia, an autosomal dominant condition and has used a mouse model. The cornea, the eye’s first avascular and transparent optical surface, is covered by a stratified epithelium that constantly renews itself; any disruption compromises transparency and threatens vision. The t...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-01 | PXD070553 | Pride
Tear proteomics Wound healing Pax6 Aniridia 
ProteomicsGalectin-3 binds isoform- and glycoform-specific lacritin in tear fluid
Galectin-3 (Gal-3) is a carbohydrate-binding protein which plays crucial roles in inflammation, immune response, cell migration, autophagy, and signaling. At the ocular surface, Gal-3 is also known to crosslink transmembrane mucins across the epithelial cell glycocalyx, forming lattice structures im...
ORGANISM(S): Homo sapiens (Human) 
2026-04-22 | PXD071066 | Pride
Lc-ms/ms Galectin-3 Tear fluid 
ProteomicsTear proteome revealed association of S100A family proteins and mesothelin with thrombosis in elderly patients with retinal vein occlusion
Tear samples collected from patients with central retinal vein occlusion (n=58) and group of healthy volunteers (n=30) were analyzed using a proteomic label-free absolute quantitative ap-proach. We found a large proportion (458 proteins with a frequency > 0.6) of tear proteome shared between healthy...
ORGANISM(S): Homo sapiens (Human) 
2022-12-13 | PXD037021 | Pride
Retinal vein occlusion  retinal hemostasis  s100a protein  mesothelin  inflammation 
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