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Stable-isotope labeling strategies are extensively used for multiplex quantitative proteomics. Hybrid isotope labeling strate-gies that combine the use of isotopic mass difference labeling and isobaric tags can greatly increase sample multiplexity. In this work, we present a novel hybrid isotope lab...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024780 | Pride
To uncover the variation in protein abundances during 48 hours under light and dark cycles (12:12-hours), we used quantitative proteomics, with TMT 6-plex isobaric labeling. We queried the S. elongatus proteome at ten different time points spanning a single 24 hour period, leading to 20 time points ...
ORGANISM(S): Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) 
2014-04-02 | PXD000510 | Pride
We used mass spectrometry-based proteomics to unravel anaplastic lymphoma kinase (ALK) signaling in the ALK and MYCN amplified neuroblastoma cell line, NB1. We specifically measured the ALK phosphoproteome upon siRNA depletion of ALK and upon ALK inhibition using the ALK-targeting small-molecule inh...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD009477 | Pride
We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a s...
ORGANISM(S): Homo sapiens 
We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a s...
ORGANISM(S): Homo sapiens 
We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a s...
ORGANISM(S): Homo sapiens 
Proteomic studies to check the effect of MEK1/2 and KRAS G12C inhibitors in lung cancer cells
ORGANISM(S): Homo sapiens (Human) 
2023-08-22 | PXD024023 | Pride
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