NC or A4GNT+THP-1 cells were incubated with TFF2-3xFlag protein overnight at 4℃, then, co-immunoprecipitation with flag beads. Elute fluid was seperated on SDS-PAGE,and Silver Staining were used to screen the differential protein bands , then,the differential protein band was excised and further ide...
To elucidate the molecular mechanism by which A4gnt-null mice develop gastric adenocarcinoma, gastric mucosa was isolated from the stomachs of wild-type and A4gnt-null mice, and microarray analysis was performed.
To elucidate the molecular mechanism by which A4gnt-null mice develop gastric adenocarcinoma, gastric mucosa was isolated from the stomachs of wild-type and A4gnt-null mice, and microarray analysis was performed. Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the ...