Sort   by:  
 Page size 
ProteomicsAbsolute quantification of proteins and histone modifications in Drosophila embryos reveals stoichiometric relationships in chromatin regulation
Gene transcription in eukaryotes is regulated through dynamic interactions of a variety of different proteins with DNA in the context of chromatin. Here, we used mass spectrometry for absolute quantification of the nuclear proteome during two stages of Drosophila embryogenesis. Before proteomics ana...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-01 | PXD015214 | Pride
Absolute proteome quantification/ histone modification quantification Prc2 H3k27 methylation Drosophila 
ProteomicsAbsolute quantification of proteins and histone modifications in Drosophila embryos
Mass spectrometry analysis for absolute and relative quantification of the nuclear proteome and of methyl-marks on selected lysine residues in histone H3 during two stages of Drosophila embryogenesis: 2-4 hr and 14-16 hr old.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-01 | PXD015306 | Pride
Histone modification Qexactive-hf Drosophila 
ProteomicsProteomes and Histone Crotonylation Analysis Reveals the Importance of Crotonylation Low Temperature in Chrysanthemum
Chrysanthemum is a garden plant with good economic benefit and high ornamental value. Chrysanthemum in the key period of flowering in autumn and winter, vulnerable to cold damage, affecting the normal growth of the chrysanthemum plant and even death. little is known regarding the study of histone cr...
ORGANISM(S): Dracocephalum grandiflorum 
2021-01-04 | PXD010297 | Pride
Proteome chrysanthemum  crotonylation 
ProteomicsComparison of different sample preparation protocols reveals lysis buffer specific extraction biases in gram-negative bacteria and human cells
We evaluated different in-solution and FASP-based sample preparation strategies for absolute protein quantification. Label-free quantification (LFQ) was employed to compare different sample preparation strategies in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK) and note...
ORGANISM(S): Homo sapiens (Human) Pseudomonas aeruginosa PAO1 
2015-10-06 | PXD001511 | Pride
Hek293 Pseudomonas aeruginosa 
ProteomicsTime-resolved Global and Chromatin Proteomics during Herpes Simplex Virus (HSV-1) Infection
Herpes simplex virus (HSV-1) lytic infection results in global changes to the host cell proteome and the proteins associated with host chromatin. We present a system level characterization of proteome dynamics during infection by performing a multi-dimensional analysis during HSV-1 lytic infection o...
ORGANISM(S): Homo sapiens (Human) Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1) 
2017-02-10 | PXD005467 | Pride
Post-translational modifications (ptms) Viral life cycle Chromatin dynamics Herpes simplex virus (hsv-1) infection Lytic infection Phosphoproteomics Histone modifications 
ProteomicsFission yeast Caprin protein is required for efficient heterochromatin establishment.
Heterochromatin is a key feature of eukaryotic genomes that serves important regulatory and structural roles in regions such as centromeres. In fission yeast, maintenance of existing heterochromatic domains relies on positive feedback loops involving histone methylation and non-coding RNAs. However,...
ORGANISM(S): Schizosaccharomyces pombe 927 
2025-02-14 | PXD050937 | Pride
Caprin Shotgun proteomics Schizosaccharomyces pombe Rna Heterochromatin Label-free quantitation 
ProteomicsIdentify proteins at Foxp3 bound cis-regulatory elements in situ
We hypothesize that nuclear factors co-occupying the genetic elements with regulatory T (Treg) cell lineage–specifying factor Foxp3 play critical roles in transcriptional regulation of Treg immune suppression function, thus, offering a unique approach to investigate the factors and their mechanisms ...
ORGANISM(S): Mus musculus (Mouse) 
2024-06-08 | PXD019050 | Pride
Proximity ligation Proteomics Foxp3 
ProteomicsMS-based proteomics of Adeonvirus type 2 infected primary human cells
To combat virus infections, which are major human killers, a deeper understanding of how viruses reprogram their hosts to create optimal production of progeny is needed. Most knowledge on the regulation of cellular gene expression during adenovirus infection is derived from studies of mRNA expressio...
ORGANISM(S): Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2) Homo sapiens (Human) 
2016-12-20 | PXD004095 | Pride
Mass spectrometry Deregulation of host cell proteins Adenovirus type 2 infection Silac Imr-90 
ProteomicsProteasome-dependent truncation of the negative heterochromatin regulator Epe1 mediates antifungal resistance.
Epe1 histone demethylase restricts H3K9-methylation-dependent heterochromatin, preventing it from spreading over, and silencing, gene-containing regions in fission yeast. External stress induces an adaptive response allowing heterochromatin island formation that confers resistance on surviving wild-...
ORGANISM(S): Schizosaccharomyces pombe 927 
2022-10-13 | PXD030205 | Pride
Fission yeast Epe1 Shotgun proteomics. Anti-fungal resistance H3k9-methylation Proteasome Heterochromatin 
ProteomicsThe epigenetic reader Phf2 is positioned in the genome by cohesin
Eukaryotic genomes are assembled into chromatin by histones and extruded into loops by cohesin. These mechanisms control important genomic functions, but whether histones and cohesin cooperate in genome regulation is poorly understood. Here we identify Phf2, a member of the Jumonji-C family of histo...
ORGANISM(S): Mus musculus (Mouse) 
2024-12-18 | PXD057926 | Pride
Cohesin Genome regulation Phf2 Epigenetic reader 
Sort   by:  
 Page size