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ProteomicsProtein precipitation of diluted samples with acetone leads to higher protein recovery and reproducibility in comparison with TCA/acetone approach
Proteomic approaches are extremely valuable in many fields of research, where mass spectrometry methods have gained an increasing interest especially due to the ability to perform quantitative analysis. Nonetheless, sample preparation prior to mass spectrometry analysis is of the utmost importance. ...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-04-25 | PXD003653 | Pride
Protein precipitation  acetone  tca/acetone  swath 
ProteomicsRapid and quantitative precipitation of a yeast proteome
Protein precipitation has applications as a front-end preparation strategy for proteome analysis, as well as depleting proteins in serum for small molecule analysis or for commercial preparation of bulk protein. The highly variable conditions previously implemented to precipitate proteins are reflec...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-23 | PXD015674 | Pride
Acetone Temperature Bottom-up Throughput Salt 
UnknownModified TCA/acetone precipitation of plant proteins for proteomic analysis.
Not available
S-EPMC6296544 | biostudies-literature
UnknownAn Efficient and Economical N-Glycome Sample Preparation Using Acetone Precipitation.
Not available
S-EPMC9786591 | biostudies-literature
UnknownSolvent Fractionation and Acetone Precipitation for Crude Saponins from Eurycoma longifolia Extract.
Not available
S-EPMC6480193 | biostudies-literature
ProteomicsZASP：A Robust Method for Proteomics Sample Preparation
ZASP can effectively remove SDS, SDC, TritonX-100 and other denaturing reagents. • ZASP has short precipitation time, high protein recovery and identification number. • Compared with acetone and SP3 precipitation method, ZASP has remarkable performance. • ZASP is particularly suitable for complex sa...
ORGANISM(S): Homo Sapiens Mus Musculus 
PXD042276 |
ProteomicsQuinoa seed proteome - Characterization of quinoa seed proteome combining different protein precipitation techniques: Improvement of knowledge of nonmodel plant proteomics
A shotgun proteomics approach was used to characterize the quinoa seed proteome. To obtain comprehensive proteomic data from quinoa seeds three different precipitation procedures were employed: MeOH/CHCl3/ddH2O, acetone either alone or with trichloroacetic acid; the isolated proteins were then in-so...
ORGANISM(S): Caryophyllales 
2016-06-24 | PXD001631 | Pride
Plant proteomics High resolution mass spectrometry Quinoa proteins- chenopodium quinoa Non-model plants Protein precipitation 
UnknownAn Optimized Trichloroacetic Acid/Acetone Precipitation Method for Two-Dimensional Gel Electrophoresis Analysis of Qinchuan Cattle Longissimus Dorsi Muscle Containing High Proportion of Marbling.
Not available
S-EPMC4404140 | biostudies-literature
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture proteins, with subse...
ORGANISM(S): Homo sapiens (Human) 
2022-08-04 | PXD028736 | Pride
Proteomics Solvent precipitation Sample preparation 
ProteomicsPalmer et al: Dental Method Comparison
The dental calculus proteome from human remains is a considered a valuable archaeological resource for studying the diet, oral microbiome, health/disease of individuals and occupation activities individuals within past societies/civilisations. The combination of advancements in LC/MS instrument sens...
ORGANISM(S): Homo sapiens (Human) 
2021-02-19 | PXD023399 | Pride
Beta-lactoglobulin Orbi-trap Archaeologica dairy proteinl Edta Deamidation Mass spectrometry Qe Dental calculus proteome Sp3 Casein Hcl Acetone precipitation Ultrafiltration 
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