Sort   by:  
 Page size 
ProteomicsCombining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
We present an approach that relies on the affinity enrichment of a target protein from a complex mixture, followed by a UV-induced activation of incorporated diazirine photo-reactive amino acids (photo-methionine and photo-leucine) and a covalent fixation of interaction partners. The captured protei...
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005349 | Pride
Pkd2 Affinity enrichment Protein interaction Photo-cross-linking Photo-methionine Photo-leucine Orbitrap fusion 
UnknownAccurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).
Not available
S-EPMC4288248 | biostudies-literature
ProteomicsLectin-based affinity enrichment and characterization of N-glycoproteins from the human tear film by mass spectrometry
Glycosylation of proteins is one of the most common post-translational modification (PTM) and plays important regulatory functions in diverse biological processes such as protein stability or cell signaling. Accordingly, glycoproteins are also a consistent part of the human tear film proteome mainta...
ORGANISM(S): Homo sapiens (Human) Sus scrofa domesticus (domestic pig) 
2023-01-25 | PXD038280 | Pride
N-glycoproteins Mass spectrometry Pngase f Tear film Multi-lectin column 
ProteomicsCOQ4 Affinity Enrichment Mass Spectrometry
Affinity enrichment mass spectrometry experiment to identify protein-protein interactions of hCOQ4-FLAG WT and hCOQ4-FLAG D164A.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-10-26 | MSV000093201 | MassIVE
GenomicsMediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit
Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recomb...
ORGANISM(S): Homo sapiens 
2012-02-24 | E-GEOD-33887 | biostudies-arrayexpress
Proteomicscocotide affinity enrichment mass spectrometry
We used affinity enrichment mass spectrometry to identify the protein binding partner of cocotide tc1 using human HaCaT cell lysate
ORGANISM(S): Homo Sapiens (human) 
2026-04-13 | PXD062883 | JPOST Repository
ProteomicsIdentification of salivary autoantibodies as biomarkers of oral cancer with IgA enrichment combined with affinity mass spectrometry
Globally, oral cavity squamous cell carcinoma (OSCC) is one of the most common fatal illnesses. Its high mortality is ascribed to the fact that the disease is often diagnosed at a late stage, which indicates an urgent need for approaches for the early detection of OSCC. The use of salivary autoantib...
ORGANISM(S): Homo sapiens (Human) 
2025-06-04 | PXD036897 | Pride
Autoantibody Proteomics Saliva Biomarker Oral cavity squamous cell carcinoma 
ProteomicsHighly accurate protein complex retrieval by affinity enrichment MS rather than affinity purification MS
Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identifi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-03-26 | PXD000955 | Pride
Ap-ms Ae-ms Yeast Interaction proteomics Label-free quantification 
UnknownAffinity-free enrichment and mass spectrometry analysis of the ovarian cancer biomarker CA125 (MUC16) from patient-derived ascites.
Not available
S-EPMC7785687 | biostudies-literature
UnknownLectin-Based Affinity Enrichment and Characterization of N-Glycoproteins from Human Tear Film by Mass Spectrometry.
Not available
S-EPMC9864693 | biostudies-literature
Sort   by:  
 Page size