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ProteomicsProtocol for high-yield production of photo-leucine-labeled proteins in Escherichia coli
Cross-linking mass spectrometry (XL-MS) is an emerging technique to obtain structural information of biomacromolecules and their complexes in cells and in vitro. In particular, certain photo-reactive amino acids (pA) such as photo-leucine (pLeu) and photo-methionine can provide unique short-distance...
ORGANISM(S): Escherichia coli 
2020-05-26 | PXD017584 | Pride
High-cell density culture Uv cross-linking Photo-leucine Molecular chaperones Mass spectrometry 
ProteomicsIncorporation of photo-amino acids in HeLa cells
In order to assess the efficiency of photo-labeling, total HeLa cell lysates treated with photo-amino acids were subjected to proteome analysis.
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005346 | Pride
Hela cells Incorporation Photo-methionine Photo-leucine Orbitrap fusion 
UnknownPhoto-leucine incorporation reveals the target of a cyclodepsipeptide inhibitor of cotranslational translocation.
Not available
S-EPMC2574519 | biostudies-literature
ProteomicsCombining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
We present an approach that relies on the affinity enrichment of a target protein from a complex mixture, followed by a UV-induced activation of incorporated diazirine photo-reactive amino acids (photo-methionine and photo-leucine) and a covalent fixation of interaction partners. The captured protei...
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005349 | Pride
Pkd2 Affinity enrichment Protein interaction Photo-cross-linking Photo-methionine Photo-leucine Orbitrap fusion 
ProteomicsAlphaLink: bringing protein structure prediction in situ with in-cell photo-crosslinking mass spectrometry, crosslinking mass spectrometry, XL-MS,
The protein structure prediction problem has been revolutionised by AlphaFold2, an algorithm that uses neural networks and evolutionary information to predict accurate models from the primary sequence. However, some proteins remain difficult to predict. Moreover, proteins are dynamic entities that e...
ORGANISM(S): Escherichia Coli 
2023-02-22 | PXD036833 | JPOST Repository
UnknownAnalysis of nidogen-1/laminin γ1 interaction by cross-linking, mass spectrometry, and computational modeling reveals multiple binding modes.
Not available
S-EPMC4227867 | biostudies-literature
ProteomicspLink Characterizing the dynamic oligomerization of HSP90β using photo-cross-linking
Cross-linking mass spectrometry (XL-MS) is a transformative tool for probing protein structures. While conventional chemical crosslinkers exhibit well-defined chemistry for specific residues, photo-crosslinkers, despite their superior reactivity, have been hindered by incomplete mechanistic understa...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD066889 | Pride
Cleavable cross-linkers Diazirine Photo-cross-linking Machine learning (decision tree) Hsp90β 
UnknownProtein structure prediction with in-cell photo-crosslinking mass spectrometry and deep learning.
Not available
S-EPMC10713450 | biostudies-literature
UnknownPrecise In Situ Delivery of a Photo-Enhanceable Inflammasome-Activating Nanovaccine Activates Anticancer Immunity.
Not available
S-EPMC11565167 | biostudies-literature
ProteomicsCharacterizing the dynamic oligomerization of Hsp90β using photo-cross-linking
Cross-linking mass spectrometry (XL-MS) is a powerful tool for probing protein structures. While conventional chemical cross-linkers react with specific residues with defined chemistry, photo-cross-linkers, despite their superior reactivity, have been hindered by incomplete mechanistic understanding...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD072009 | Pride
Hsp90 Cleavable cross-linkers Diazirine Photo-cross-linking 
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