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ProteomicsCombining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
We present an approach that relies on the affinity enrichment of a target protein from a complex mixture, followed by a UV-induced activation of incorporated diazirine photo-reactive amino acids (photo-methionine and photo-leucine) and a covalent fixation of interaction partners. The captured protei...
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005349 | Pride
Pkd2 Affinity enrichment Protein interaction Photo-cross-linking Photo-methionine Photo-leucine Orbitrap fusion 
ProteomicsIncorporation of photo-amino acids in HeLa cells
In order to assess the efficiency of photo-labeling, total HeLa cell lysates treated with photo-amino acids were subjected to proteome analysis.
ORGANISM(S): Homo sapiens (Human) 
2017-04-10 | PXD005346 | Pride
Hela cells Incorporation Photo-methionine Photo-leucine Orbitrap fusion 
UnknownPartial Destabilization of Amyloid-β Protofibril by Methionine Photo-Oxidation: A Molecular Dynamic Simulation Study.
Not available
S-EPMC10035002 | biostudies-literature
UnknownInvestigating the Role of Antioxidant Compounds in Riboflavin-Mediated Photo-Oxidation of Methionine: A 1H-NMR Approach.
Not available
S-EPMC7558052 | biostudies-literature
UnknownResponse Surface Methodology Approach to Evaluate the Effect of Transition Metals and Oxygen on Photo-Degradation of Methionine in a Model Wine System Containing Riboflavin.
Not available
S-EPMC9937536 | biostudies-literature
UnknownPhoto-Methionine, Azidohomoalanine and Homopropargylglycine Are Incorporated into Newly Synthesized Proteins at Different Rates and Differentially Affect the Growth and Protein Expression Levels of Auxotrophic and Prototrophic E. coli in Minimal Medium.
Not available
S-EPMC10380393 | biostudies-literature
ProteomicsProtocol for high-yield production of photo-leucine-labeled proteins in Escherichia coli
Cross-linking mass spectrometry (XL-MS) is an emerging technique to obtain structural information of biomacromolecules and their complexes in cells and in vitro. In particular, certain photo-reactive amino acids (pA) such as photo-leucine (pLeu) and photo-methionine can provide unique short-distance...
ORGANISM(S): Escherichia coli 
2020-05-26 | PXD017584 | Pride
High-cell density culture Uv cross-linking Photo-leucine Molecular chaperones Mass spectrometry 
ProteomicsMACSPI Enables Tissue-Selective Proteomic and Interactomic Analyses in Multicellular Organisms
Multicellular organisms are composed of many tissue types that have distinct morphologies and functions, which are largely driven by specialized proteomes and interactomes. To define the proteome and interactome of a specific type of tissue in an intact animal, we developed a localized proteomics ap...
ORGANISM(S): Homo sapiens (Human) 
2024-05-02 | PXD048632 | Pride
Interactomic Macspi Proteomic Photo-ana 
UnknownRadiation- and Photo-Induced Oxidation Pathways of Methionine in Model Peptide Backbone under Anoxic Conditions.
Not available
S-EPMC8125225 | biostudies-literature
ProteomicsA binding site hotspot map of the FKBP12–rapamycin–FRB ternary complex by photo-affinity labeling and mass spectrometry-based proteomics
Structural characterization of small molecule binding site hotspots within the global proteome is uniquely enabled by photo-affinity labeling (PAL) coupled with chemical enrichment and unbiased analysis by mass spectrometry (MS). MS-based binding site hotspot maps provide structural resolution of in...
ORGANISM(S): Homo sapiens (Human) 
2024-01-04 | PXD013420 | Pride
Photo-affinity labeling Natural product Structural proteomics 
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