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We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. These experiments were performed with 0, 1, 2, 4, 8, 16 and 13 h of chase. Data from 3 biological replicates ar...
ORGANISM(S): Mus musculus (Mouse) 
2016-10-06 | PXD004929 | Pride
SILAC- and AHA-labelling analysis of Raw264.7 cells infected with MNV-1 at MOI=10TCID50/cell and harvested at 6.5 and 10.5 hours post infection. AP-MS enrichment of AHA-labelled nascent polypeptides represents the first quantitative neoproteomics analysis of norovirus infected cells.
ORGANISM(S): Mus musculus (Mouse) 
2021-07-02 | PXD025169 | Pride
mRNA-seq analysis of RAGE knock-in (RageAHA/AHA) and RAGE-/- mice
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo sapiens (Human) 
2016-10-06 | PXD004915 | Pride
Cell surface heparan sulfate (HS) plays an essential role in RAGE signaling by inducing RAGE oligomerization. To understand the physiological significance of HS-induced RAGE oligomerization in vivo, we generated RAGE knock-in mice (RageAHA/AHA) by introducing point mutations to specifically disrupt ...
ORGANISM(S): Mus musculus 
2021-05-11 | GSE174178 | GEO
modENCODE_submission_2937 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
modENCODE_submission_3069 This submission comes from a modENCODE project of Michael Snyder. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We are identifying the DNA binding sites for 300 transcription factors in C. elegans. Each transcription factor gene is ta...
ORGANISM(S): Caenorhabditis elegans 
Development of an improved workflow for newly synthesized proteome analysis, based on combined pulsed metabolic labelling with L-azidohomoalanine (AHA) and semi-automated click chemistry-based enrichments with magnetic alkyne agarose beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD036886 | Pride
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