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ProteomicsProject title: Endogenous AIRE-immunoprecipitation from primary human neutrophils
Autoimmune regulator (AIRE) is a transcriptional regulator. We here assess the role for AIRE in primary human neutrophils. We found that the functional role for AIRE in neutrophils is to regulate the extrinsic apoptotic pathway involving the Fas/TNFR death receptors and Cathepsin G and the expressio...
ORGANISM(S): Homo sapiens (Human) 
2024-01-25 | PXD047989 | Pride
Neutrophil Aire Human 
TranscriptomicsChIP to AIRE regulated genes in HEK 293 cells
The Autoimmune Regulator (AIRE) protein is expressed in thymic medullary epithelial cells, where it promotes the ectopic expression of tissue-restricted antigens needed for efficient negative selection of developing thymocytes. Mutations in AIRE cause APECED syndrome, which is characterized by a bre...
ORGANISM(S): Homo sapiens 
2010-09-10 | E-GEOD-17216 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression profile of medullary thymic epithelial cells - mTEC 3.10 strain - submitted of in vitro Aire knockdown - siRNA silencing-
The oligo microarrays were used to determine microRNA expression profiles of medullary thymic epithelial cells (mTECs) submitted of in vitro Aire Knockdown (siRNA silencing).
ORGANISM(S): Mus musculus 
2012-08-21 | E-MEXP-3359 | biostudies-arrayexpress
TranscriptomicsDifferential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells
Promiscuous gene expression (pGE) of numerous self-antigens in thymic epithelial cells (TEC) enables the elimination of self-reactive T cells. The autoimmune regulator (Aire) is the only known molecular determinant driving pGE in the thymus but the existence of Aire-independent mechanisms has been i...
ORGANISM(S): Mus musculus 
2015-05-19 | E-GEOD-65617 | biostudies-arrayexpress
TranscriptomicsExpression data from WT and Aire deficient mTECs
Aire is an important transcription regulator that mediates a role in central tolerance via promoting the promiscuous expression of tissue-specific antigens in the thymus. Although several mouse models of Aire-deficiency have been described, none has analysed the phenotype induced by a mutation that ...
ORGANISM(S): Mus musculus 
2009-03-15 | E-GEOD-14365 | biostudies-arrayexpress
TranscriptomicsHEK293 stably expressing Autoimmune regulator protein
Auto Immune REgulator (AIRE) protein expression in HEK293 cells Three cell-lines where used in the study: HEK293-NC; control cell-line expressing yellow fluorescent protein (YFP) HEK-AIRE1, HEK-AIRE2; two different clone-derived Autoimmune regulator protein expressing cell-lines HEK-AIRE-D312A; c...
ORGANISM(S): Homo sapiens 
2010-05-05 | E-GEOD-16877 | biostudies-arrayexpress
ProteomicsProtein microarray-based autoantibody screening in 51 APS1 patients and 21 healthy controls
Autoimmune polyendocrine syndrome type 1 (APS1) is a monogenic disorder that features multiple autoimmune disease manifestations. It is caused by mutations in the Autoimmune regulator (AIRE) gene, which promote thymic display of thousands of peripheral tissue antigens in a process critical for estab...
ORGANISM(S): Homo sapiens 
2019-06-27 | E-MTAB-8097 | biostudies-arrayexpress
TranscriptomicsExpression data from WT and Aire KO thymic medullary epithelial cells (MECs)
The aim of this study is to analyze the transcriptional effects of Aire deficiency in the thymus, using the Affymetrix MoGene platform to analyze variation in exon usage MECs were isolated from 4-6 wk-old WT or Aire KO ((B6xNOD)F1 background) mice. Three WT and three Aire-KO mice taken individually ...
ORGANISM(S): Mus musculus 
2011-11-23 | E-GEOD-33878 | biostudies-arrayexpress
TranscriptomicsThe effect of the Autoimmune regulator (Aire) gene on mRNA and lncRNA transcriptional profile of mTEC 3.10 cell line
In this study, we used the murine (Mus musculus) medullary thymic epithelial cell line (mTEC 3.10 cell line) co-cultured with fresh thymocytes as a functional assay for mTEC-thymocyte adhesion. Then we analyzed the differential transcriptional profile of this cell line, by means of Agilent oligo mic...
ORGANISM(S): Mus musculus 
2021-12-31 | E-MTAB-6541 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type and aire -/- murine meduallary thymic epithelial cells to study the immunological roles of aire protein in thymus.
Mice used were B6/129 F2's, 3-5 weeks of age, either wild type or with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluo...
ORGANISM(S): Mus musculus 
2007-12-15 | E-GEOD-85 | biostudies-arrayexpress
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