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MetabolomicsDysbiosis of Gut Microbiota and Metabolomic Alterations in Myasthenia Gravis: Insights from 16S rRNA Sequencing and Untargeted Metabolomics

Background: Myasthenia gravis (MG) is an autoimmune disorder of neuromuscular transmission. Gut dysbiosis has been implicated in autoimmune pathogenesis, yet integrated microbial and metabolomic profiling in MG remains scarce.

Objectives:  To characterize gut microbiota and t...

2026-04-13 | MTBLS14266 | MetaboLights
ProteomicsMS-based sequencing of the anti-FLAG-M2 antibody
We demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by LC-MS/MS in a bottom-up fashion. Furthermore, we apply a dual fragmentation...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-11 | PXD023419 | Pride
Antibody Stepped hcd Ethcd De novo sequencing 
TranscriptomicsA Highly Sensitive and Unbiased Approach for Elucidating Antibody Repertoires
Developing B lymphocytes undergo V(D)J recombination to assemble germline V, D, and J gene segments into exons that encode the antigen-binding variable region of immunoglobulin (Ig) heavy (H) and light (L) chains. IgH and IgL chains associate to form the B cell receptor (BCR), which upon antigen bi...
ORGANISM(S): Mus musculus 
2016-07-22 | E-GEOD-82126 | biostudies-arrayexpress
ProteomicsDe novo sequencing of antibody light chain proteoforms from patients with multiple myeloma
In multiple myeloma diseases, monoclonal immunoglobulin light chains (LCs) are abundantly produced, with as a consequence in some cases the formation of deposits affecting various organs, such as kidney, while in other cases to remain soluble up to concentrations of several g.L-1 in plasma. The exac...
ORGANISM(S): Homo sapiens (Human) 
2021-08-11 | PXD025884 | Pride
Proteoforms Top-down proteomics Antibody Bottom-up proteomics De novo sequencing Light chain disease 
TranscriptomicsRNA-seq of human peripheral blood mononuclear cells following oral cholera vaccination
In this study we performed RNA-seq to identify human transcriptomic signatures associated with antibody responses to an oral cholera vaccine. We isolated total RNA from peripheral blood mononuclear cells of vaccinated volunteers who following two priming oral immunisations, had either strong or no a...
ORGANISM(S): Homo sapiens 
2021-03-23 | E-MTAB-9069 | biostudies-arrayexpress
ProteomicsDe Novo Sequencing of Polyclonal Antibodies by Integrating Intact Mass, Top-Down and Bottom-Up Mass Spectrometry
Antibodies are specialized proteins produced by the adaptive immune system to identify and neutralize harmful antigens. Antibody-based diagnostics and therapeutics have advanced rapidly, with recombinant antibodies derived from monoclonal antibodies (mAbs) becoming the preferred standard due to thei...
ORGANISM(S): Homo sapiens (Human) 
2025-10-31 | PXD063526 | Pride
Intact mass De novo sequencing Top-down Bottom-up Polyclonal antibody sequencing 
ProteomicsBenchmarking and integrating human B-cell receptor genomic and antibody proteomic profiling
Antibodies are immune glycoproteins that represent a crucial part of the adaptive immune system as they mediate broad protection against viruses, bacteria, and cancer. Moreover, antibodies may serve as a unique source of information on past and current physiological and pathological events in the hu...
ORGANISM(S): Homo sapiens (Human) 
2024-06-04 | PXD046237 | Pride
Human Lc-msms Plasma Antibody 
ProteomicsDeep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite de novo Protein Sequencing by Mass Spectrometry
Label-free protein sequencing is critically enabled by bottom-up, mass spectrometry-based proteomics workflows. Applications such as antibody sequencing or antigen discovery require de novo reconstruction of peptide and protein sequences. While trypsin has long served as the gold-standard protease i...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD063988 | Pride
Hta-proteases Hybrid fragmentation by eacid De novo antibody sequencing Bottom-up proteomics 
ProteomicsSequencing the anti-MUC1 hybridoma antibody 139H2
Mucin 1 (MUC1) is a transmembrane mucin expressed at the apical surface of epithelial cells at different mucosal surfaces including breast and intestine. The MUC1 extracellular domain contains a variable number of tandem repeats (VNTR) of 20 amino acids, which are heavily O-linked glycosylated. Mono...
ORGANISM(S): Mus musculus (Mouse) 
2024-03-18 | PXD043489 | Pride
Muc1 Antibody Hybridoma De novo 
GenomicsGenetic measurement of memory B-cell recall using antibody repertoire sequencing

We developed an improved high throughput sequencing approach to measure the quantities and sequences of the repertoire of antibody heavy chain RNA in a blood sample. Using this approach we analyzed the antibody repertoire in response to yearly vaccinations with influenza vaccines TIV and LAIV in ...

phs000656.v1.p1 | EGA
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