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UnknownModeling contaminants in AP-MS/MS experiments.
Not available
S-EPMC4494835 | biostudies-literature
TranscriptomicsJanus-Like Opposing Roles of CD47 in Autoimmune Brain Inflammation in Human and Mice
Gene transcripts and proteins expressed during disease pathogenesis identify targets for therapy. We performed microarray analysis of histologically characterized multiple sclerosis (MS) brain lesions in comparison with control brain samples to identify differentially expressed molecules. We identif...
ORGANISM(S): Homo sapiens 
2012-06-23 | E-GEOD-38010 | biostudies-arrayexpress
ProteomicsNicotiana benthamiana AP-MS for TSWV NSs
Despite the advancement of structural and functional research on TSWV NSs, its protein-interacting targets in host plants are still largely unknown. In this research, we investigated the NSs-interacting proteins in Nicotiana benthamiana via affinity purification and mass spectrometry (AP-MS) analysi...
ORGANISM(S): Nicotiana benthamiana 
2021-01-04 | PXD022401 | Pride
Tswv nss Ap-ms Nicotiana benthamiana 
ProteomicsMapping ISG15 sites on GAPDH and PGK1 by AP-MS
We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological...
ORGANISM(S): Homo sapiens (Human) 
2026-03-02 | PXD071724 | Pride
Ap-ms Pgk1 Gapdh Isg15 Glycolysis 
UnknownAccurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).
Not available
S-EPMC4288248 | biostudies-literature
UnknownBioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data.
Not available
S-EPMC7029486 | biostudies-literature
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidics-based (AP-MS) workflow to identify PPIs usi...
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD012800 | Pride
Interaction proteomics Low input Ap-ms 
ProteomicsOn-chip AP-MS: Interaction proteomics on a microfluidics platform
Here we present a microfluidics-based affinity purification-mass spectrometry (AP-MS) pipeline to identify protein-protein interactions (PPI) using minute amounts of input material. The use of this automated platform allowed us to identify the human cohesin and CCC complex from only 4 micrograms of ...
ORGANISM(S): Homo sapiens (Human) 
2022-03-10 | PXD011323 | Pride
Interaction proteomics Low input Ap-ms 
UnknownAn AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations.
Not available
S-EPMC5864832 | biostudies-literature
ProteomicsIdentify the interacting proteins of FBXL4 by AP-MS
Affinity purification coupled with mass spectrometry (AP-MS) is powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex.Mass spectrometry was performed to identify the interacting proteins of FBXL4 fro...
ORGANISM(S): Homo sapiens (Human) 
2024-05-12 | PXD040601 | Pride
Human Fbxl4 Ap-ms 
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