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ProteomicsFiber-associated microbiota protects against diet-induced amelioration of the colonic mucus barrier
Here we have shown that diet-mediated alterations of the gut microbiota composition cause an erosion of the colonic mucus barrier. A compensatory increase in cellular mucus production by the host is not sufficient to re-establish the barrier, possibly due to a lacking increase in mucus secretion. Wh...
ORGANISM(S): Mus musculus (Mouse) 
2017-12-22 | PXD006129 | Pride
Gut microbiota Obesity Fiber Mucus layer Metabolic disease Barrier function Western diet Diet-microbe-host interaction 
TranscriptomicsMulti-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth rates
Background Lactococcus lactis is recognised as a safe (GRAS) microorganism and has hence gained interest in numerous biotechnological approaches. As it is fastidious for several amino acids, optimization of processes which involve this organism requires a thorough understanding of its metabolic reg...
ORGANISM(S): Lactococcus lactis subsp. lactis Il1403 
2011-01-11 | E-GEOD-26536 | biostudies-arrayexpress
TranscriptomicsTranscriptional pattern of E. coli BW25113 and its ptsA, glpK and dhaKLM isogenic mutants growing on DHA (dihydroxyacetone).
Dihydroxyacetone (DHA) is an attractive molecule produced in a wide range of industries . DHA is found among all the kingdoms as an intermediate of various metabolic pathways and can be used as a carbon source by many organisms. The bacterium Escherichia coli is able to grow on DHA as the sole carbo...
ORGANISM(S): Escherichia coli K-12 
2019-06-03 | E-MTAB-7666 | biostudies-arrayexpress
ProteomicsLactococcus lactis protein turnover
Triplicate chemostat cultivations were carried out with L. lactis subsp. lactis IL1403 at specific growth rates 0.1 and 0.5 h-1 on a chemically defined medium, where both, glucose and lysin (Lys) were jointly limiting the growth. After reaching the steady physiological state, medium was switched to ...
ORGANISM(S): Lactococcus lactis subsp. lactis Il1403 
2014-01-28 | PXD000494 | Pride
Lc-msms Lactococcus lactis Protein turnover 
UnknownMulti-omics approach to study the growth efficiency and amino acid metabolism in Lactococcus lactis at various specific growth rates.
Not available
S-EPMC3049130 | biostudies-literature
ProteomicsComparison and applications of label-free absolute proteome quantification methods on Escherichia coli
Three different label-free proteome quantification methods - APEX, emPAI and iBAQ - were evaluated to measure proteome-wide protein concentrations in the cell. All the methods were applied to a sample from Escherichia coli chemostat culture. A Pearson squared correlation of approximately 0.6 among t...
ORGANISM(S): Escherichia coli 
2013-07-24 | PXD000283 | Pride
Ups2 Shotgun proteomics Ibaq E. coli 
ProteomicsIL-22 promotes postnatal formation of MUC17 glycocalyx barrier that protects host enterocytes
The apical aspects of absorptive epithelial cells form a highly organized brush border membrane (BBM), shaped by densely packed microvilli that are coated with a glycocalyx. Here, we present evidence showing that the glycocalyx forms an epithelial barrier that prevents exogenous molecules from gaini...
ORGANISM(S): Mus musculus (Mouse) 
2021-02-16 | PXD018592 | Pride
Innate immunity Mucin Intestine Microvilli Enterocyte Muc13 Microbiota Muc17 Glycocalyx 
ProteomicsIleum and colon goblet cell proteome
This study is a comparative proteomics study of FACS sorted Muc2 expressing and non expressing cells in mouse ileum and colon.
ORGANISM(S): Mus musculus (Mouse) 
2021-04-19 | PXD011527 | Pride
Ileum Proteomics Goblet cells Colon 
ProteomicsProteomic analysis of neonatal rats distal colon mucus
Compositional analysis of the neonatal rats distal colon mucus proteome by label-free mass spectrometry indicated significant variability in mucus protein composition at different ages.
ORGANISM(S): Rattus norvegicus (Rat) 
2025-04-01 | PXD052791 | Pride
Mass-spectrometry Lfq proteomics Rats Mucus Colon 
ProteomicsProteomics of Muc17-deficient mice small intestine epithelial cells
To evaluate the impact of Muc17 deletion on other major intestinal mucins, we employed proteomic analysis of isolated Si5 epithelial cells fromMuc17fl/fl and Muc17∆IEC cohoused littermates. Deletion of Muc17 did not result in a compensatory change in the abundance of the gel-forming mucin Muc2 and m...
ORGANISM(S): Mus musculus (Mouse) 
2025-05-06 | PXD048896 | Pride
Muc17  muc3  small intestine  epithelial cells  lfq 
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