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ProteomicsKCMF1 links RAD6 to UBR4 and lysosome-mediated degradation
Raw mass spec. data files, converted peak lists and TPP results files for KCMF1 links RAD6 to UBR4 and lysosome-mediated degradation by Hong et. al, MCP 2014. RAD6 is a ubiquitin E2 protein with roles in a number of different biological processes. Here, using affinity purification coupled with mass ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-01-01 | MSV000078983 | MassIVE
ProteomicsDiscovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6
Cellular selectivity of a chemical probe for the UBD of HDAC6. A biotinylated analog of HDAC6 chemical probe SGC-UBD253 bound to streptavidin beads was used to enrich HDAC6 from cytoplasmic fraction of HEK293 lysate. A triplicate of each condition - competition with probe, control, DMSO - was done.
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD039880 | Pride
Ubiquitin binding domain Chemical probe Ubd Chemoproteomics Hdac6 
ProteomicsPeptide mapping of purified huntingtin protein samples from different eukaryotic expression systems
Huntingtin expression, purification and biophysical characterisation: purified huntingtin protein samples of different polyQ lengths derived from insect cell and mammalian cell production were digested and subject to peptide mass spectrometry.
ORGANISM(S): Spodoptera frugiperda Homo sapiens (Human) 
2019-03-12 | PXD010865 | Pride
Pbacmam Post-translational modification Huntingtin Huntington’s disease Polyglutamine Eukaryotic expression system Htt Hd Polyq 
ProteomicsChemoproteomics of an NSD2-PWWP1 Chemical Probe
Here we use competitive chemoproteomics pulldowns followed by label-free quantitative LC-MS/MS to assess target engagement and selectivity profiles of UNC6934 and UNC7145, a chemical probe targeting the PWWP1 domain of NSD2 and its negative control counterpart, respectively. To this end, we used a b...
ORGANISM(S): Homo sapiens (Human) 
2021-08-06 | PXD017641 | Pride
Epigenetics Chemoproteomics Histone methyltransferase Chemical probe 
ProteomicsGID4-dependent proteome and interactome
The CTLH complex is a multi-subunit ubiquitin ligase complex that recognizes substrates with Pro/N-degrons via substrate receptor GID4. Recently, focus has turned to the complex as a potential mediator of targeted protein degradation, but the role GID4-mediated substrate ubiquitylation and proteasom...
ORGANISM(S): Homo sapiens (Human) 
2024-05-16 | PXD038487 | Pride
Human Bioid Lc-ms/ms 
TranscriptomicsRNA-seq of murine small intestinal organoids treated with type I PRMT inhibitor MS023 against untreated controls
Murine small intestinal organoids were cultured in the presence or absence of 5µM inhibitor MS023 (PubChem CID 92136227), which targets type I protein arginine methyltransferases Prmt1, Prmt3, Prmt4, Prmt6, Prmt8. Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), medi...
ORGANISM(S): Mus musculus 
2020-12-16 | E-MTAB-9291 | biostudies-arrayexpress
TranscriptomicsRNA-seq of murine small intestinal organoids treated with Ep300/Crebbp inhibitor I-CBP112 against untreated controls
Murine small intestinal organoids were cultured in the presence or absence of 3µM inhibitor I-CBP112 (PubChem CID 90488984), which targets E1A Binding Protein P300 (Ep300) and Creb-binding protein (Crebbp, Cbp). Organoids were grown in culture media containing EGF, Noggin and R-spondin (ENR), media ...
ORGANISM(S): Mus musculus 
2020-12-16 | E-MTAB-9290 | biostudies-arrayexpress
ProteomicsSzewczyk_InhibitionOfPRMT7_P36_2020
This dataset consists of 16 raw MS files, acquired on Orbitrap Fusion Lumos Tribrid mass spectrometer operated in Data Dependent Acquisition (DDA) mode for the 8 input files and DDA with simultaneous parallel reaction monitoring (targeted masses; 599.33, 604.33, 608.23, 606.33, 407.89, 403.22, 651.3...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-08 | MSV000084773 | MassIVE
OtherAssessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Data from ProteomeXchange, PXD ID: PXD002534. File: GR_130129_20_OT3_NT_L3MBTL2_FAB_31_G11_2_HEK293.mzXML. Published as part of Nat Methods. 2015 Jun 29 . From the Abstract: {{i}} ... We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320016573 | GPMDB
Resource Reanalysis 
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