Sort   by:  
 Page size 
ProteomicsBrain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons
Supporting TMT MS data for paper (doi: 10.1016/j.neuron.2021.12.029) by Goldsmith J. et al., titled "Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons". Index of RAW files uploaded: AO3068-3079: Fractionated (12 fr...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2023-08-01 | MSV000092565 | MassIVE
TranscriptomicsTranscriptional regulation by Pho23 modulates the frequency of autophagosome formation.
Autophagy as a conserved degradation and recycling machinery is important in normal development and physiology, and defects in this process are linked to many kinds of disease. Because too much or too little autophagy can be detrimental, the process must be tightly regulated both temporally and in m...
ORGANISM(S): Saccharomyces cerevisiae 
2014-04-24 | E-GEOD-57031 | biostudies-arrayexpress
Proteomicsproximity-proteomics-based autophagosome content profiling
proximity-proteomics-based autophagosome content profiling to identify a role for LC3C in maintaining basal mitochondrial homeostasis. Selected mitochondrial proteins, including MTX1, were targeted by LC3C and p62 through a piecemeal mitophagy pathway.
ORGANISM(S): Homo sapiens (Human) 
2018-02-28 | PXD008177 | Pride
Hela Orbitrap elite Proxiity labeling 
UnknownProteomic profiling of autophagosome cargo in Saccharomyces cerevisiae.
Not available
S-EPMC3953483 | biostudies-literature
ProteomicsAutophagosome content profiling in TBK1-E696K knockin MEFs
To determine how the mutant TBK1-E696K protein impacts autophagosomes, we performed autophagosome content profiling using protease protection coupled APEX2 proximity proteomics of autophagosomes of homozygous TBK1-E696K knockin and wiltype mouse embryonic fibroblasts (MEFs) transfected with a APEX2-...
ORGANISM(S): Homo sapiens (Human) 
2024-03-16 | PXD048795 | Pride
Substrates Mefs Autophagy E696k Tbk1 
UnknownThe HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17.
Not available
S-EPMC3982997 | biostudies-literature
ProteomicsAutophagosome content profiling - STAR Protocol Data Set
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autopha...
ORGANISM(S): Homo sapiens (Human) 
2021-04-06 | PXD024335 | Pride
Human Apex2 Proximity proteomics Proteinase k digest 
ProteomicsQuantitative proteomics profilling of p62 droplet
Phase separated p62 body plays pivotal roles during autophagosome formation, however, the underlying mechanisms are still not fully understood. Mass spectrometry-based proteomics revealed significant enrichment of vesicle trafficking components within p62 bodies.
ORGANISM(S): Homo Sapiens Rattus Norvegicus 
2023-06-09 | PXD038262 |
ProteomicsCargo and lipid profiling of isolated autophagosomes upon basal autophagy, starvation, and proteasome inhibition
Autophagy is responsible for degradation of an extensive portfolio of cytosolic cargoes that are engulfed in autophagosomes to facilitate their transport to lysosomes. Besides basal autophagy, which constantly degrades cellular material, the pathway is dynamically altered by different conditions, re...
ORGANISM(S): Homo sapiens (Human) 
2022-10-06 | PXD024419 | Pride
Hela Autophagy Autophagosome Label free quantification 
ProteomicsA conserved membrane curvature-generating protein is crucial for autophagosome formation in fission yeast
Organelles are shaped by curvature-generating proteins, which include the reticulons and REEPs that are involved in forming the endoplasmic reticulum (ER). A conserved REEP subfamily differs from the ER-shaping REEPs in abundance and membrane topology and has unidentified functions. Here, we show th...
ORGANISM(S): Schizosaccharomyces pombe 927 
2023-08-06 | PXD043260 | Pride
Reep1 Autophagy Fission yeast 
Sort   by:  
 Page size