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ProteomicsIdentification of the covalent binding site of Ibrutinib and 5Z-7-Oxozeaenol to ZAK
The goal of this experiment was to identify the reaction site of Ibrutinib and 5Z-7-Oxozeaenol to ZAK by determination of mass shifts according to a covalent modification by the inhibitors using mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2017-10-13 | PXD007752 | Pride
Ibrutinib 5z-7-oxozeaenol Cys150 Recombinant zak Cys22 Covalent modification 
ProteomicsDifferential kinobeads selectivity profiling
The goal of this project was to use differential kinobeads profiling for the discrimination of reversible and irreversible kinase targets of different covalent kinase inhibitors. For this, differences in target engagement measured in a lysate- and a cell-based kinobeads assay were compared in a kino...
ORGANISM(S): Homo sapiens (Human) 
2017-10-13 | PXD007920 | Pride
Ibrutinib Kinobeads Ramos b-lymphocyte Cell-based assay 5z-7-oxozeaenol Saturated ibrutinib A549 lung epithelia cell line Lysate-based assay Acalabrutinib Cc-292 Lc-ms/ms 
ProteomicsConfirmation of ZAK as covalent target of Ibrutinib
The goal of this experiment was the confirmation of ZAK as a covalent target of Ibrutinib by an affinity enrichment experiment with a linkable analogue of Ibrutinib.
ORGANISM(S): Homo sapiens (Human) 
2017-10-13 | PXD007638 | Pride
Lysate-based assay Linkable ibrutinib affinity enrichment Sds elution vs bead trypsinationlc-ms/ms Ibrutinib Ramos b-lymphocyte lysate 
ProteomicsInterval-based secretomics unravels the time-dependent acute phase response in hepatocyte model systems
Mass spectrometry based secretomics approaches preferentially utilize serum-free culture conditions to circumvent serum induced interference and enable sufficient analytical depth. However, this negatively affects a wide range of cellular functions and thus reduces the biological relevance. Moreover...
ORGANISM(S): Homo sapiens (Human) 
2022-05-04 | PXD027821 | Pride
Il1b Heparg Shedding Secretomics Liver Acute-phase response Interval-based secretomics Il6 Hepg2 
ProteomicsSystematic analysis of protein turnover in primary cells - Part IV
To better understand proteostasis in health and disease, determination of protein half-lives is essential. We improved the precision and accuracy of peptide-ion intensity based quantification in order to enable accurate determination of protein turnover in non-dividing cells using dynamic-SILAC. Thi...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD008512 | Pride
Hepatocytes Isobarquant Silac Protein half lives 
ProteomicsSystematic analysis of protein turnover in primary cells - Part VI
To better understand proteostasis in health and disease, determination of protein half-lives is essential. We improved the precision and accuracy of peptide-ion intensity based quantification in order to enable accurate determination of protein turnover in non-dividing cells using dynamic-SILAC. Thi...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD008516 | Pride
Isobarquant Silac Thp1 cells Protein half lives 
ProteomicsSystematic analysis of protein turnover in primary cells - Part II
To better understand proteostasis in health and disease, determination of protein half-lives is essential. We improved the precision and accuracy of peptide-ion intensity based quantification in order to enable accurate determination of protein turnover in non-dividing cells using dynamic-SILAC. Thi...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD008515 | Pride
Isobarquant Silac Primary nk cells Protein half lives 
ProteomicsSystematic analysis of protein turnover in primary cells - Part I
To better understand proteostasis in health and disease, determination of protein half-lives is essential. We improved the precision and accuracy of peptide-ion intensity based quantification in order to enable accurate determination of protein turnover in non-dividing cells using dynamic-SILAC. Thi...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD008511 | Pride
Isobarquant Silac Primary b cells Protein half lives 
ProteomicsSystematic analysis of protein turnover in primary cells - Part III
To better understand proteostasis in health and disease, determination of protein half-lives is essential. We improved the precision and accuracy of peptide-ion intensity based quantification in order to enable accurate determination of protein turnover in non-dividing cells using dynamic-SILAC. Thi...
ORGANISM(S): Homo sapiens (Human) 
2018-06-08 | PXD008513 | Pride
Isobarquant Silac Monocyte Protein half lives 
ProteomicsSystematic analysis of protein turnover in primary cells - Part V
To better understand proteostasis in health and disease, determination of protein half-lives is essential. We improved the precision and accuracy of peptide-ion intensity based quantification in order to enable accurate determination of protein turnover in non-dividing cells using dynamic-SILAC. Thi...
ORGANISM(S): Mus musculus (Mouse) 
2018-06-08 | PXD008514 | Pride
Isobarquant Silac Protein half lives Primary neuron cells 
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