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MetabolomicsUntargeted LC-MS metabolomics of RANKL-differentiated Bone Marrow–Derived Macrophages ± Bacillus Calmette–Guérin (BCG) exposure

To investigate the metabolites and pathways modulated by BCG in RANKL-differentiated macrophages, thereby elucidating the metabolic mechanisms underlying BCG-induced macrophage activation, BMMs were stimulated with 50 ng/mL RANKL for 5 days to generate osteoclast-like cells. Subsequently, one gro...

2026-03-31 | MTBLS12992 | MetaboLights
TranscriptomicsDissecting the Mycobacterium bovis BCG response to macrophage infection to identify targets for anti-tuberculosis drug and vaccine discovery
New strategies are required to reduce the worldwide burden of tuberculosis. Intracellular survival and replication of Mycobacterium tuberculosis after macrophage phagocytosis is a fundamental step in the complex host-pathogen interactions that lead to granuloma formation and disease. Greater underst...
ORGANISM(S): Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2 
2022-01-14 | E-MTAB-11107 | biostudies-arrayexpress
TranscriptomicsMycobacterium bovis-BCG vaccination induces a specific pulmonary transcriptome biosignature in mice
In the present study, we applied microarray technology to define a biosignature from the whole genome expression in lung and spleen samples after BCG vaccination and M. bovis infection of BALB/c mice. The aims were two-fold, namely to define biosignatures that could predict vaccine success before ch...
ORGANISM(S): Mus musculus 
2010-07-29 | E-GEOD-21149 | biostudies-arrayexpress
TranscriptomicsDosR-regulon genes induction in Mycobacterium bovis BCG under aerobic conditions
In this report we demonstrated that under aerobic conditions, Mycobacterium bovis BCG expressing an hsp60-driven second copy of the hypoxia-related transcriptional regulator DosR increased 2-fold or greater the expression of 38 out of the 48 genes belonging to the DosR regulon, including the latency...
ORGANISM(S): Mycobacterium tuberculosis 
2010-05-20 | E-GEOD-21932 | biostudies-arrayexpress
ProteomicsMS analysis of BCG and recombinant BCG "70C-RBD"
BCG and recombinant BCG "70C-RBD" were cultured in Sauton media. Culture filtrate and cells lysate were separated by SDS-PAGE. The bands at 40kDa and 60kDa were excised and proteins were reduced and alkylated. In-gel digestion (Trypsin) was performed. The samples were analyzed by TripleTOF5600+ conj...
ORGANISM(S): Cellular Organisms 
2023-12-07 | PXD046929 | JPOST Repository
Proteomics4D Label-Free Quantitative Proteomics of Six BCG vaccine Strains (2 Groups with 3 Biological Replicates Each)
Investigation of the Types and Expression Levels of Target Proteins Regulated by the Transcription Factor NapR Following Its Overexpression in Intracellular BCG vaccine
ORGANISM(S): Mycobacterium bovis BCG 
2025-02-28 | PXD059682 | Pride
Proteomics Transcription factor Bcg vaccine 
TranscriptomicsIdentification of antigens presented by MHC for vaccines against tuberculosis
The immune response against tuberculosis relies, at least in part, on CD4+ T cells. Protective vaccines require the induction of antigen-specific CD4+ T cells via mycobacterial peptides presented by MHC class-II in infected macrophages. We have purified MHC class-I and MHC-II peptides and analysed t...
ORGANISM(S): Mycobacterium tuberculosis variant bovis BCG 
2020-01-08 | E-MTAB-8057 | biostudies-arrayexpress
ProteomicsExploring the potential role of moonlighting function of the surface-associated proteins from Mycobacterium bovis BCG Moreau and Pasteur by comparative proteomic
Tuberculosis (TB) is one of major causes of death worldwide. Bacillus Calmette-Guerin (BCG) is the only licensed TB vaccine and its inability to protect against adult pulmonary TB can be due to genetic differences among strains described since the 1940s. In this work, we compared the proteomic profi...
ORGANISM(S): Mycobacterium bovis BCG str. Moreau RDJ 
2019-05-15 | PXD006141 | Pride
Moonlighting function Surface-associated proteins Mycobacterium bovis bcg moreau 
TranscriptomicsThe innate and adaptive immune response to BCG stimulation in splenocytes taken from C57BL/6 mice
The aim of this experiment was to investigate differential gene expression in splenocytes stimulated with BCG from naïve and BCG vaccinated mice. The differences between naïve and BCG vaccinated mice might indicate the mechanisms by which BCG vaccination confers an enhanced ability of splenocytes fr...
ORGANISM(S): Mus musculus 
2013-06-01 | E-GEOD-42090 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of five strains of Mycobacterium bovis in order to understand the evolution, attenuation and variable protective efficacy of BCG vaccines
Data is also available from http://bugs.sgul.ac.uk/E-BUGS-43
ORGANISM(S): Mycobacterium bovis 
2007-03-20 | E-BUGS-43 | biostudies-arrayexpress
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