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ProteomicsProximal Biotinylation Based Combinatory Approach for Isolating Plasma Membrane Proteins
Comprehensive profiling of the cell surface proteome has been challenging due to the lack of tools for an effective and reproducible way to isolate plasma membrane proteins from mammalian cells. Here, we employ a proximity-dependent biotinylation approach to label and isolate plasma membrane protein...
ORGANISM(S): Homo sapiens (Human) 
2020-08-28 | PXD018383 | Pride
Sucrose density fractionation Cell surface Biotinylation Plasma membrane 
ProteomicsThiol-cleavable Biotin for Chemical and Enzymatic Biotinylation and its Application to Mitochondrial TurboID Proteomics
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-beads digestion in numerous biological applications. However, the popular on-beads digestion method faces major challenges of streptavidin contamination, the lost information of bioti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-21 | MSV000087256 | MassIVE
ProteomicsBIOSITe: A novel method for direct detection of site specific biotinylation
Proximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family protei...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-12-19 | PXD007862 | Pride
Bioid Proximity dependent biotinylation Bcr abl Mass spectrometry Ip Peptide Biotinylation Biosite 
ProteomicsDecreasing Protein Biotinylation Background in a Diatom Facilitates Proximity Labeling of the Periplastidial Compartment Proteome
Diatoms are ecologically and industrially significant microalgae, crucial for global carbon fixation and biotechnological applications. Their complex plastid membrane structures, resulting from secondary endosymbiosis, remain poorly characterized, particularly the periplastidial compartment (PPC). P...
ORGANISM(S): Homo Sapiens Oryza Sativa Nicotiana Benthamiana Nannochloropsis Oceanica Thalassiosira Pseudonana Arabidopsis Thaliana Phaeodactylum Tricornutum 
2025-03-14 | PXD061818 |
ProteomicsProfiling the interactome of oligonucleotide drugs by proximity biotinylation
Drug-ID applies proximity biotinylation to identify drug-protein interactions inside living cells. The covalent conjugation of a drug molecule with a biotin ligase enables targeted biotinylation of the drug-bound proteome and its identification by SILAC-MS/MS. We investigate the interactome of two w...
ORGANISM(S): Homo sapiens (Human) 
2024-01-09 | PXD045992 | Pride
Antisense oligonucleotide Basu Proximity biotinylation Drug protein interactome Live cell Biotin ligase Hdac inhibitors Silac In situ biotinylation Bet inhibitors Bioid 
ProteomicsProtocol for cell surface biotinylation of magnetic labeled and captured human peripheral blood mononuclear cells
Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs)....
ORGANISM(S): Homo sapiens (Human) 
2022-11-08 | PXD038027 | Pride
Macs Surface proteome Biotinylation 
ProteomicsSuper-resolution proximity labeling method with enhanced direct identification of biotinylation sites
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein-protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemented, in-depth identification of specific biotin...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047979 | Pride
Biotinylation site Apex2 Turboid Lc-ms Membrane protein topology Bioid Proximity labeling 
ProteomicsIdentification of the Tau aggresome in progressive supranuclear palsy by in situ biotinylation by antibody recognition
Here, we used an unbiased proteomic approach, which combines antibody-mediated proximity-detection of co-aggregated proteins together with mass spectrometry. Biotinylation by antibody recognition (BAR) is a recently developed method, by which a primary antibody recognises the target of interest in f...
ORGANISM(S): Homo sapiens (Human) 
2023-02-27 | PXD028237 | Pride
Tauopathy  aggresome  biotinylation 
UnknownA proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues.
Not available
S-EPMC8748630 | biostudies-literature
ProteomicsWithin-host adaptive phenotypic responses of bovine Staphylococcus aureus
The Gram-positive bacterium S. aureus is one of the most common causes of contagious bovine mastitis. A predominant S. aureus clone persists within its host over a long period. In the present study, we compared the surface proteome of two isolates, collected from a naturally infected dairy cow with ...
ORGANISM(S): Staphylococcus aureus 
2021-08-14 | PXD025013 | Pride
Chronic bovine mastitis Surface shaving Biotinylaton Host adaption Staphylococcus aureus Cell surface proteome 
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