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GenomicsLRF genome-wide occupancy in CD4+ T cells
To examine whether LRF protein could bind cis-regulatory elements in the genes that LRF regulates. We performed Chromatin immunoprecipitation followed by deep-sequencing (ChIPseq) on activated T cells using an in vivo biotinylation and streptavidin pull-down approach.
ORGANISM(S): Mus musculus 
2022-03-30 | GSE149993 | GEO
ProteomicsWAGO-4 Localization to Germ Granules Is Important for Its Accurate Small RNA Loading
There were several MS experiments used in this study: 1) Proximity labeling of the P granules 2) Protein levels estimation from whole worm lysates and 3) wago-4 KO expression check (PRM analysis). 1) Proximity labeling strategy was used to estimate P granule composition in 3xFG mutant (FG domains of...
ORGANISM(S): Caenorhabditis elegans 
2025-10-14 | PXD062557 | Pride
C. elegans Proximity labeling Fg repeats P granules 
TranscriptomicsRNA-biotin based pull down assay with polyA site (PAS) RNA substrates in Hela Nuclear Extract followed by RNA-seq to discover trans-acting RNA molecules involved in mRNA 3 processing
We aimed to discover trans-acting RNA molecules involved in mRNA 3 processing. We reasoned that, if there exist such functional RNAs, they must directly associate with the key machinery responsible for mRNA 3 processing. Therefore, it would be of great value to comprehensively identify RNAs interact...
ORGANISM(S): Homo sapiens 
2017-06-01 | E-MTAB-5383 | biostudies-arrayexpress
UnknownAn effective plasma membrane proteomics approach for small tissue samples.
Not available
S-EPMC4456939 | biostudies-literature
UnknownProtocol for identification of cell-surface proteins with horseradish peroxidase.
Not available
S-EPMC12757472 | biostudies-literature
ProteomicsDiscovery of TRPV1-Associated Molecular Chaperones via PafA-Mediated Proximity Labeling
To identify TRPV1-associated molecular chaperones, we applied a PafA-based proximity labeling system using both N- and C-terminal TRPV1 fusions. Three groups were co-transfected with BCCP-Pup(E) for in-cell biotinylation. Biotinylated proteins were validated by immunofluorescence and Western blot, f...
ORGANISM(S): Mus Musculus 
PXD066698 |
ProteomicsAn effective plasma membrane proteomics approach for small tissue samples
Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective e...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-22 | PXD002227 | Pride
Orbitrap velos Affinity-purification Mice Acute slice biotinylation assay Membrane proteomics Shotgun proteomics 
ProteomicsGrowth factor-induced alterations of plasma membrane-associated proteome of DAOY cell
In this study we investigated alterations in the plasma membrane (PM)-associated proteome of DAOY medulloblastoma cells. We used a surface biotinylation approach to label PM-associated proteins. We enriched for biotinylated proteins by streptavidin-pull-down and determined the composition by mass-sp...
ORGANISM(S): Homo sapiens (Human) 
2022-03-14 | PXD030597 | Pride
Map4k4 Medulloblastoma Hepatocyte growth factor Plasma membrane proteome 
UnknownBioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).
Not available
S-EPMC7415991 | biostudies-literature
ProteomicsLabel-free LC-MS/MS analysis of proteins associated with specific RNAs in HeLa cells
Proteins co-localizing with RNA targets of interest in HeLa cells were identified using our recently optimized hybridization-proximity labeling approach (HyPro2) combined with label-free mass spectrometry. Briefly, fixed and permeabilized cells were hybridized with digoxigenin-labeled antisense olig...
ORGANISM(S): Homo sapiens (Human) 
2025-08-04 | PXD063191 | Pride
Actb Rna-protein interactions Lncrna Fus Pre-mrna Hybridization-proximity labeling Pnctr. 
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