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ProteomicsVirotrap-based interactome analysis of a NTR protein
Ribosome profiling has revealed translation outside of canonical coding sequences (CDSs) including translation of short upstream ORFs, long non-coding RNAs, overlapping ORFs, ORFs in UTRs or ORFs in alternative reading frames. Studies combining mass spectrometry, ribosome profiling and CRISPR-based ...
ORGANISM(S): Homo sapiens (Human) 
2022-07-12 | PXD030216 | Pride
Virotrap Protein-protein interaction Lc-ms/ms 
ProteomicsN-terminal COFRADIC on cytosolic proteins of HEK293T cells
Ribosome profiling revealed translation outside of canonical coding sequences (CDSs) including translation of short upstream open-reading frames (ORFs), long non-coding RNAs, ORFs in UTRs or ORFs in alternative reading frames. Ribo-seq but also bioinformatics-based prediction and RNA-sequencing repo...
ORGANISM(S): Homo sapiens (Human) 
2022-07-12 | PXD030601 | Pride
Cytosol Hek293t cells N-terminal peptides 3 proteases Cofradic Atis Lc-ms/ms 
ProteomicsMapping the cytosolic proteins of HEK293T cells
N-terminal proteoforms stem from the same gene but differ at their N-terminus, and most of these are found to be truncated, though some are N-terminally extended caused by ribosomes starting translation from codons in the annotated 5’UTR, and/or carry modified N-termini different from those of the c...
ORGANISM(S): Homo sapiens (Human) 
2023-06-08 | PXD039127 | Pride
Cytosol Fractionation Hek293t cells Lc-ms/ms 
ProteomicsN-terminal COFRADIC on cytosolic proteins of HEK293T cells - UniProt search
N-terminal proteoforms stem from the same gene but differ at their N-terminus, and most of these are found to be truncated, though some are N-terminally extended caused by ribosomes starting translation from codons in the annotated 5’UTR, and/or carry modified N-termini different from those of the c...
ORGANISM(S): Homo sapiens (Human) 
2023-06-08 | PXD039392 | Pride
Cytosol Hek293t cells N-terminal peptides 3 proteases Cofradic Atis Lc-ms/ms 
ProteomicsVirotrap-based interactome analysis of 20 pairs of N-terminal proteoforms and their canonical protein
N-terminal proteoforms stem from the same gene but differ at their N-terminus, and most of these are found to be truncated, though some are N-terminally extended caused by ribosomes starting translation from codons in the annotated 5’UTR, and/or carry modified N-termini different from those of the c...
ORGANISM(S): Homo sapiens (Human) 
2023-06-08 | PXD039171 | Pride
Virotrap Protein-protein interaction N-terminal proteoform Lc-ms/ms 
ProteomicsAP-MS-based interactome analysis of three pairs of N-terminal proteoforms and their canonical protein
N-terminal proteoforms stem from the same gene but differ at their N-terminus, and most of these are found to be truncated, though some are N-terminally extended caused by ribosomes starting translation from codons in the annotated 5’UTR, and/or carry modified N-termini different from those of the c...
ORGANISM(S): Homo sapiens (Human) 
2023-06-08 | PXD039085 | Pride
Protein-protein interaction N-terminal proteoform Ap-ms Flag tagged Lc-ms/ms 
ProteomicsCheck of selected NTR peptide spectra by comparison with synthetic peptides
Ribosome profiling has revealed translation outside of canonical coding sequences (CDSs) including translation of short upstream ORFs, long non-coding RNAs, overlapping ORFs, ORFs in UTRs or ORFs in alternative reading frames. Studies combining mass spectrometry, ribosome profiling and CRISPR-based ...
ORGANISM(S): Homo Sapiens 
2022-08-02 | PXD030285 | panorama
ProteomicsUBR4 KO analysis with TMT labeling
The impact of NatC and UBR4 on the human proteome (protein abundance). Analysis of HAP1 WT siCtr, HAP1 WT siUBR4, HAP1 NAA30 KO siCtr, and HAP1 NAA30 KO siUBR4 cells.
ORGANISM(S): Homo sapiens (Human) 
2023-09-20 | PXD034410 | Pride
Hap1 ko cells Tmt Ubr4 Lc-ms/ms 
ProteomicsImpact of NatC on N-terminal acetylome
The impact of NatC subunits NAA30, NAA35 and NAA38 on the human N-terminal acetylome. Analysis of HAP1 WT, NAA30 KO, NAA35 KO and NAA38 KO cells
ORGANISM(S): Homo sapiens (Human) 
2023-09-13 | PXD034992 | Pride
Lc-ms/ms Hap cells Natc Scx N-terminal enrichment 
ProteomicsLFQ shotgun proteomic analysis of HAP1 NatC KO cells
The impact of NatC subunits NAA30, NAA35 and NAA38 on the human proteome (protein abundance). Analysis of HAP1 WT, NAA30 KO, NAA35 KO and NAA38 KO cells
ORGANISM(S): Homo sapiens (Human) 
2023-09-20 | PXD034104 | Pride
Hap1 cells Natc ko Lfq Shotgun Lc-ms/ms 
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