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ProteomicsThe periplasmic oxidoreductase DsbA is required for virulence of the phytopathogen Dickeya solani
The DsbA oxidoreductase is a crucial factor responsible for introduction of disulfide bonds to the extracytoplasmic proteins in bacteria. A lack of the proper disulfides frequently leads to instability and/or loss of protein function. In pathogens, numerous envelope and extracellular proteins play i...
ORGANISM(S): Dickeya solani 
2022-02-15 | PXD028047 | Pride
Dickeya solani Swath-ms Dsba Lc-ms/ms 
ProteomicsProteomic study on the lymphocytes from pregnant Wistar rats females treated with immunosuppressive regimen
Kidney transplantation remains the optimal therapeutical option for end stage kidney disease. Current immunosuppressive regimens are efficient in combating acute kidney rejection. However, the insight in chronic kidney allograft injury remains limited. Simultaneously, pregnancy in kidney recipients ...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-11 | PXD035571 | Pride
Rat Lymphocyte Swath-ms 
ProteomicsQuantitative proteomic analysis of fresh-frozen human breast tissue and tumor samples using microLC-SWATH-MS
Future improvements in the field of breast cancer prevention, diagnosis and treatment require deeper knowledge and better understanding of the molecular processes and signaling pathways that lead to the transformation of normal cells to cancer ones and the disease progression. Quantitative proteome ...
ORGANISM(S): Homo sapiens (Human) 
2026-03-16 | PXD008408 | Pride
Breast tissue Fresh frozen Breast tumor Microlc Swath-ms Quantitative proteomics 
ProteomicsProteomic analysis of filaggrin insufficient keratinocytes and secreted exosomes.
Filaggrin (FLG) is a key structural protein expressed in epidermal keratinocytes. Mutations in the filaggrin gene (FLG) are strongly linked to atopic dermatitis (AD) and allergic inflammation occurring later in life at distant body locations in AD patients. Keratinocytes secrete exosomes, small, lip...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD026859 | Pride
Exosomes Cd1a Keratinocyte Ichthyosis vulgaris Atopic dermatitis Filaggrin Lc-ms/ms 
ProteomicsProteomic analysis of small extracellular vesicles isolated from human blood plasma
Exosomes are small, lipid-rich membrane vesicles secreted by all nucleated cells. These vesicles carry a variety of cargo including nucleic acids, proteins and lipids. Due to their size and flexibility, exosomes can enter the circulation and therefore serve as means of communication between distant...
ORGANISM(S): Homo sapiens (Human) 
2023-06-07 | PXD036724 | Pride
Exosomes Extracellular vesicles Blood plasma 
ProteomicsCompatibility of distinct label-free proteomic workflows in absolute quantification of proteins linked to the oocyte quality in human follicular fluid (the Triple-Quad TOF workflow)
We present a comprehensive analysis of human follicular fluid proteome by comparing several strategies of sample preparation, mass spectrometry analysis, and data processing, resulting in the identification of 1638 proteins. We devised two separate label-free quantitative workflows, termed after the...
ORGANISM(S): Homo sapiens (Human) 
2021-07-23 | PXD024223 | Pride
Lc-ms/ms Oocyte quality control Human follicular fluid Oocyte maturity Total protein approach Swath-ms Proteome Blastocyst development 
ProteomicsCompatibility of distinct label-free proteomic workflows in absolute quantification of proteins linked to the oocyte quality in human follicular fluid (the Quad-Orbitrap workflow)
We present a comprehensive analysis of human follicular fluid proteome by comparing several strategies of sample preparation, mass spectrometry analysis, and data processing, resulting in the identification of 1638 proteins. We devised two separate label-free quantitative workflows, termed after the...
ORGANISM(S): Homo sapiens (Human) 
2021-07-23 | PXD024347 | Pride
Lc-ms/ms Oocyte quality control Human follicular fluid Oocyte maturity Total protein approach Proteome Blastocyst development 
ProteomicsHuman follicular fluid proteomic and peptidomic composition quantitative studies by SWATH-MS methodology. Applicability of high pH RP-HPLC fractionation
Analysis of proteomic composition of human follicular fluid (hFF) has been proposed as a potential tool of oocyte quality evaluation. In order to develop an efficient method to investigate the hFF proteome and peptidome components, we applied a prefractionation scheme of hFF material consisting of ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-19 | PXD008073 | Pride
Human Follicular fluid Fractionation Swath-ms 
ProteomicsLabel-free quantitative SWATH-MS proteomic analysis of adult myocardial slices in vitro after biomimetic electromechanical stimulation
Conventional in vitro cell cultures are relatively available and have been widely used in explaining many mechanisms, but their simple construction is not directly applied to the in vivo environment. A possible solution to overcome this problem is special in vitro model maintained with cardiac slice...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-08-25 | PXD034891 | Pride
Rat Heart Myocardial slices Swath-ms 
ProteomicsProteome and peptidome of human follicular fluid
Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small scale study three samples from four donors (12 samples in total) were analyzed to determine wh...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006550 | Pride
Human Follicular fluid Swath Lc-ms/ms 
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