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Proteomicsnascent proteome analysis of HCT116 TP53 wild type and TP53 knockout cells treated with Neocarcinostatin (NCS) for 6h
In order to investigate the potential role of TP53 in regulating translation, HCT116 wild type and TP53 knockout cells were analyzed using both RNA sequencing, Ribosome sequencing and nascent proteome analysis. The cells were treated with 0.2 µg/ml Neocarcinostatin (NCS) to induce DNA damage and act...
ORGANISM(S): Homo sapiens (Human) 
2022-04-27 | PXD029512 | Pride
Tp53 Ncs Nascent proteome Metabolic labeling Dna damage 
ProteomicsProteomic analysis of GC7-treated senescent and proliferating BJ fibroblasts
Quantitative analysis of global proteomic changes in proliferating and senescent BJ fibroblasts, treated with eIF5A hypusination inhibitor GC7 (10 uM) for 12h.
ORGANISM(S): Homo sapiens (Human) 
2024-07-12 | PXD034251 | Pride
Gc7 Eif5a hypusination 
ProteomicsNascent proteome analysis of proliferating and senescent BJ fibroblast treated with GC7
Analysis of proteomic changes in proliferating and senescent BJ fibroblast treated with eIF5A hypusination inhibitor GC7 for 12h.
ORGANISM(S): Homo sapiens (Human) 
2024-07-12 | PXD034334 | Pride
Gc7 Eif5a hypusination Nascent proteome 
ProteomicsApplying plexDIA analysis to newly synthesized proteome samples generated using a semi-automated enrichment protocol
Samples generated with a newly development semi-automated enrichment protocol for newly synthesized proteins, using different amounts of protein input, were analysed using data-independent acquisition (DIA) and processed with the plexDIA software features of DIA-NN (https://www.nature.com/articles/s...
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD043817 | Pride
Silac Plexdia Quanpa 
ProteomicsBenchmarking plexDIA analysis with SILAC-labelled Hela cell samples
SILAC labelled Hela cell lysates were mixed in defined ratios and analysed using both DDA and DIA methods, to serve as a benchmark for the novel plexDIA features of DIA-NN.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD039578 | Pride
Dia-nn Dia Hela Silac Plexdia 
ProteomicsBenchmarking of DDA and plexDIA analysis with SILAC-labelled Hela cell samples
SILAC labelled Hela cell lysates were mixed in defined ratios and analysed using both DDA and DIA methods, to serve as a benchmark for the novel plexDIA features of DIA-NN.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD039580 | Pride
Hela Silac Dda 
Proteomicssemi-automated enrichment of AHA-containing newly-synthesized proteins with magnetic alkyne agarose beads
Development of an improved workflow for newly synthesized proteome analysis, based on combined pulsed metabolic labelling with L-azidohomoalanine (AHA) and semi-automated click chemistry-based enrichments with magnetic alkyne agarose beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD036886 | Pride
Automation Silac Click chemistry Nascent proteome 
ProteomicsNewly synthesized proteome analysis of interferon gamma treated Hela cells
Using a newly-developed workflow for quantitative newly synthesized proteome analysis (QuaNPA), featuring automated sample processing and multiplexed DIA (plexDIA) analysis, changes in the newly synthesized proteome of IFN-gamma treated Hela cells were monitored over time.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD038915 | Pride
Dia Nascent proteome analysis Automated sample processing Interferon gamma Silac 
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