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TranscriptomicsTranscriptional Response to transgenic BRAF(V600E) in the intestinal epithelium
BRAF(V600E) is a frequent mutation in colon cancer. We have analysed transcriptional effects of BRAF(V600E) in the intestinal epithelium of transgenic mice that harbour an inducible BRAF(V600E) transgene. Mice used in this experiment were compound transgenic for a stem-cell specific Lgr5-EGFP Report...
ORGANISM(S): Mus musculus 
2013-12-31 | E-GEOD-50678 | biostudies-arrayexpress
TranscriptomicsHuman BRAF-V600E melanoma cell lines drug sensitive vs drug resistant
aCGH of human melanoma cell lines comparing parental (drug sensitve) vs isogenic drug resistant-derived subline Two condition experiment: two BRAF-V600E mutant cell lines (drug sensitive - parental baseline) vs two derived sublines after chronic exposure to the MEK inhibitor trametinib (drug resista...
ORGANISM(S): Homo sapiens 
2014-04-21 | E-GEOD-49430 | biostudies-arrayexpress
ProteomicsRICTOR/mTORC2 downregulation in BRAF V600E melanoma cells promotes resistance to BRAF/MEK inhibition
Background Main drawback of BRAF/MEK inhibitors (BRAF/MEKi)-based targeted therapy in the management of BRAF- mutated cutaneous metastatic melanoma (MM) is the development of therapeutic resistance. We aimed to define in this context the specific role of mTORC2, a signaling complex defined by the pr...
ORGANISM(S): Homo sapiens (Human) 
2024-06-22 | PXD050614 | Pride
Targeted therapy Rictor Braf v600e melanoma Mitochondrial metabolism Drug resistance Nampt Mtorc2 
ProteomicsProteomic analysis of BEAS-2B lung epithelial cells after the introduction of the BRAF V600E mutation using CRISPR/Cas9
To mimic the naturally occurring BRAF V600E mutation in lung cancer, a BRAF V600E knock-in BEAS-2B cell model was established using CRISPR/Cas9. The proteomics analysis was carried out to evaluate the underlying changes at the proteome level. Proteomics analysis revealed significant changes in the p...
ORGANISM(S): Homo sapiens (Human) 
2025-04-22 | PXD058027 | Pride
V600e Lung Amino acid metabolism Nsclc Braf 
ProteomicsBRAF complexes - Discrete cytosolic macromolecular BRAF complexes exhibit distinct activities and composition
BRAF interactomes dependent on the mutation V600E are analyzed by SEC-PCP-SILAC and HA-IPs comparing V600E to WT
ORGANISM(S): Homo sapiens (Human) 
2017-01-03 | PXD004585 | Pride
Ap-ms V600e Sec-pcp-silac Caco2 cells Braf Lc-ms/ms 
TranscriptomicsOncogenic BRAF regulates oxidative metabolism via PGC1alpha and MITF
We evaluated two matched human cell lines derived from primary melanocytes, termed pmel+BRAF(V600E) and pmel+BRAF(V600E)+MITF. These cells are therefore isogenic with the exception of the expression of MITF. Gene Set Enrichment Analysis of microarray data identified a highly significant induction of...
ORGANISM(S): Homo sapiens 
2013-03-07 | E-GEOD-38007 | biostudies-arrayexpress
GenomicsOncogenic KRAS(G12V) and BRAF(V600E) in intestinal organoids
Oncogenic KRAS(G12V) and BRAF(V600E) in intestinal organoids
PRJNA474218 | ENA
TranscriptomicsRNA-seq melanoma
Using a chromatin regulator-focused shRNA library, we found that suppression of sex determining region Y-box 10 (SOX10) in melanoma causes resistance to BRAF and MEK inhibitors. To investigate how SOX10 loss leads to drug resistance, we performed transcriptome sequencing (RNAseq) of both parental A...
ORGANISM(S): Homo sapiens 
2014-01-04 | E-GEOD-50535 | biostudies-arrayexpress
Genomics3' RNAseq of EGFR mutant lung cancer cell line overexpressing BRAF V600E treated with inhibitors
3' RNAseq of EGFR mutant lung cancer cell line overexpressing BRAF V600E treated with inhibitors
PRJEB47888 | ENA
GenomicsA secondary mutation in BRAF confers resistance to RAF inhibition in a BRAF V600E-mutant brain tumor
A secondary mutation in BRAF confers resistance to RAF inhibition in a BRAF V600E-mutant brain tumor
PRJNA476393 | ENA
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