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ProteomicsComplexome Profiling of the thylakoid proteome from Arabidopsis thaliana
We here present an investigation of the Arabidopsis thaliana energy metabolism taking place in the chloroplasts. Thylakoids were purified from Arabidopsis thaliana leaves and membrane bound protein complexes characterized by Blue native polyacrylamide gel electrophoresis as well as by the complexome...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-08-18 | PXD035871 | Pride
Arabidopsis thaliana Proteome Thylakoids Lc-msms Blue native 
ProteomicsComplexome Profiling of the thylakoid proteome from Viscum album
Viscum album is known for its special mode of cellular respiration. It lacks the mitochondrial NADH dehydrogenase complex (complex I of the respiratory chain) and has restricted capacities to generate mitochondrial adenosine triphosphate (ATP). We here present an investigation of the V. album energy...
ORGANISM(S): Viscum album 
2022-08-18 | PXD035825 | Pride
Mistletoe Proteome Thylakoids Lc-msms Blue native Viscum album 
ProteomicsComplexome Profiling of the mitochondrial proteome from Viscum album
Viscum album is known for its special mode of cellular respiration. It lacks the mitochondrial NADH dehydrogenase complex (complex I of the respiratory chain) and has restricted capacities to generate mitochondrial adenosine triphosphate (ATP). We here present an investigation of the V. album energy...
ORGANISM(S): Viscum album 
2023-10-24 | PXD041061 | Pride
Mitochondria Mistletoe Proteome Lc-msms Blue native Viscum album 
ProteomicsHPLC-TIMS-TOF-MS of purified mitochondrial I+III2 supercomplex from Arabidopsis thaliana
Mitochondrial I+III2 supercomplex was purified from Arabidopsis thaliana cells using differential centrifugation including Percoll step gradients and continuous sucrose gradients. Supercomplex fractions were digested with trypsin using the SP3 approach. Peptides were further purified with SepPak car...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-12-30 | PXD036482 | Pride
Arabidopsis Supercomplex Mitochondria 
ProteomicsComplexome Profiling of the mitochondrial proteome from Viscum album
Recent genomic studies revealed that mitochondria from mistletoe species appear to lack a major amount of genes, indicating a possible loss of the NADH ubiquinone oxidoreductase (complex I) which forms an essential part of the mitochondrial Oxidative Phosphorylation System (OXPHOS). This dataset is ...
ORGANISM(S): Viscum album 
2018-04-23 | PXD008974 | Pride
Mitochondria Mistletoe Proteome Lc-msms Blue native Viscum album 
ProteomicsMulti-Protease Investigation of Arabidopsis Thaliana Cell Culture Mitochondria
The aim of this project is to deeply map the proteome of mitochondria from the model plant Arabidopsis thaliana. For this purpose, mitochondria were isolated from Arabidopsis cell cultures, their proteins extracted and processed using SP3 digestion. To achieve high sequence coverage, the proteins we...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2023-08-02 | MSV000092578 | MassIVE
ProteomicsSingle organelle function and organization as estimated from Arabidopsis mitochondrial proteomics
Aiming at defining the protein content and composition of a single mitochondrion of Arabidopsis thaliana, shotgun analyses were performed on purified mitochondria from cultured heterotrophic Arabidopsis cells. Based on microscopic observations on the size of mitochondria, as well as biochemical prop...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-11 | PXD014292 | Pride
Plant mitochondria 
ProteomicsProteomics Analysis of the UMK2 Mutation in Arabidopsis thaliana seeds
We compared the proteome of dry as well as germinating seeds of Arabidopsis thaliana wild type Col-0 with the respective proteomes from an umk2 (AT4G25280) frameshift mutant. Germinating seeds were incubated for 48 hours in 1/2-MS medium in Petri dishes at 4 degree Celsius followed by 24 hours of a...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2023-07-03 | MSV000092331 | MassIVE
ProteomicsProteomic investigation of seed development under high nitrate supply in plants lacking Pyrroline-5-carboxylate dehydrogenase
Free proline is an amino acid known to have many functions in plants, particularly in stress adaptation and development. Its metabolism is well known, but its exact role in plant physiology has remained elusive. Proline catabolism is ensured by the sequential action of the enzymes Proline dehydrogen...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2024-06-10 | MSV000094979 | MassIVE
ProteomicsPPR proteins in Arabidopsis mitoribosomes
Investigation of the protein composition of mitoribosomal subunits in Arabidopsis thaliana by complexome profiling
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-04-30 | PXD011088 | Pride
Mitoribosomal subunits Ppr proteins Mitoribosomes Complexome profiling 
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