Sort   by:  
 Page size 
TranscriptomicsTranscription profiling of human adenocarcinoma cell line A549 over-expressing ELMOD2
ELMOD2 cDNA from the IMAGE clone 3897166 was cloned into the pDsRed-Monomer-N1 expression vector. ELMOD2 was overexpressed in the A549(adenomcarcinoma)cell line and cells transfected with empty vector were considered controls. Transcript profiles were compared with the Affymetrix Hgu133plus2 arrays....
ORGANISM(S): Homo sapiens 
2009-12-07 | E-MEXP-1838 | biostudies-arrayexpress
ProteomicsIdentification of TTC29 protein partners in mouse testis
We previously identified gene variants in TTC29 that induce human male infertility due to asthenozoospermia and MMAF phenotype (Lores et al. Am J hum genet 2019). By means of CRISPR/Cas9 mutagenesis we demonstrated that the orthologous TTC29 protein in mouse also constitute a flagellar protein that...
ORGANISM(S): Mus musculus (Mouse) 
2023-11-06 | PXD039854 | Pride
Co-immunoprecipitation Asthenozoospermia Male infertility Ttc29 Crispr mouse model 
ProteomicsFANCA protein-protein interaction network
The objective of the project is to determine the interactome of FANCA in order to identify the partners and the biochemical and molecular pathways to which it contributes, which will be successively functionally analyzed by bioinformatics approaches and in living cells.
ORGANISM(S): Homo sapiens (Human) 
2026-08-05 | PXD068730 | Pride
Fanconi anemia Rna splicing Dna damage response Ribosome biogenesis 
ProteomicsProteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly
The aim of this project was to identify cellular factors enriched with SARS-CoV-2 virions after affinity capture using biotinylated ACE2 receptor as compared to biotin control.
ORGANISM(S): Homo sapiens (Human) 
2024-01-22 | PXD045409 | Pride
Sars-cov-2 Virion capture Ace2 receptor Cellular factors G3bp1 and g3bp2 
ProteomicsProteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly
The aim of this project was to identify cellular factors enriched in SARS-CoV-2 virion preparations from infected Calu-3 cells as compared to preparation from mock-infected cells.
ORGANISM(S): Homo sapiens (Human) 
2024-01-22 | PXD045406 | Pride
Sars-cov-2 Virions Cellular factors G3bp1 and g3bp2 Proteomic 
ProteomicsMALT1 paracaspase controls cholesterol homeostasis in glioblastoma stem-like cells through lysosome proteome shaping
Glioblastoma stem-like cells (GSCs) compose a tumor-initiating and propagating-population, remarkably vulnerable to any variation in the stability and integrity of the endolysomal compartment. Previous work showed that the expression and activity of the paracaspase MALT1 control GSC viability via ly...
ORGANISM(S): Homo sapiens (Human) 
2025-01-16 | PXD040855 | Pride
Malt1 Srebp2 Niemann-pick type c1 Lysoip Glioblastoma Lysosomes Cholesterol 
ProteomicsMALT1 paracaspase controls cholesterol homeostasis in glioblastoma stem-like cells through lysosome proteome shaping
Glioblastoma stem-like cells (GSCs) compose a tumor-initiating and propagating-population, remarkably vulnerable to any variation in the stability and integrity of the endolysomal compartment. Previous work showed that the expression and activity of the paracaspase MALT1 control GSC viability via ly...
ORGANISM(S): Homo sapiens (Human) 
2025-01-16 | PXD040862 | Pride
Malt1 Srebp2 Niemann-pick type c1 Lysoip Glioblastoma Lysosomes Cholesterol 
TranscriptomicsTranscription profiling of Neuropeptide S Receptor 1-A (NPSR1-A) transfected human epithelial kidney cells stimulated with neuropeptide S to identify downstream targets
The purpose of this study was to identify downstream gene targets regulated by Neuropeptide S Receptor 1 (NPSR1) upon NPS stimulation. To do this we used human epithelial kidney (HEK)-293H cell lines stable transfected with NPSR1-A and stimulated with NPS for 6 hours. Two controls were used: NPS sti...
ORGANISM(S): Homo sapiens 
2006-08-24 | E-MEXP-829 | biostudies-arrayexpress
TranscriptomicsStudy of Cj0667 function in Campylobacter jejuni
In order to study the function of the Campylobacter jejuni Cj0667 gene, a series of experiments were carried out. Two strains were constructed: a Cj0667 knockout strain and a strain with a second copy over-expressing Cj0667 from an fdxA promoter. The transcriptomes of these were all compared to the ...
ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 
2012-12-31 | E-GEOD-35120 | biostudies-arrayexpress
TranscriptomicsStudy of Cj0138 function in Campylobacter jejuni
In order to study the function of the Campylobacter jejuni Cj0138 gene, a series of experiments were carried out. Three strains were constructed: a Cj0138 knockout strain, a strain where the Cj0138 knockout was complemented in trans, and a strain with a second copy over-expressing Cj0138 from an fdx...
ORGANISM(S): Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 
2012-12-31 | E-GEOD-35119 | biostudies-arrayexpress
Sort   by:  
 Page size