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ProteomicsGeLC-MS/MS analysis of cereal aphid saliva
Saliva was collected from c.40,000 diet reared aphids, pooled and concentrated. Samples were coming from 2 aphid species: Metopolophium dirhodum and Sitobion avenae. Proteins were fractionated using SDS PAGE and visible bands were subjected to in gel digestion. Tryptic peptides were analysised on a ...
ORGANISM(S): Sitobion avenae (English grain aphid) Metopolophium dirhodum (Rose grain aphid) 
2013-04-09 | PXD000113 | Pride
Saliva Metopolophium dirhodum Aphids Sitobion avenae Ltq-ft 
ProteomicsThe salivary gland proteome of root-galling grape phylloxera (Daktulosphaira vitifoliae Fitch) feeding on Vitis spp.
Herbivory plant-parasite interactions depend on the delivery of effector molecules by the invading insect species. Sedentary gall forming insects, such as grape phylloxera (Daktulosphaira vitifoliae FITCH, Phylloxeridae) secrete multiple effectors into host plant tissues that alter host cellular fun...
ORGANISM(S): Daktulosphaira vitifoliae 
2020-07-22 | PXD014663 | Pride
Daktulosphaira vitifoliae Proteomics Salivary gland Phylloxera Effectors 
ProteomicsMating precedes selective immune priming which is maintained throughout bumblebee queen diapause
Diapause is a key life-history event characterised by arrested development, suppressed metabolism and increased stress tolerance. This state allows an organism to avoid prolonged periods of harsh and inhospitable environmental conditions. For species where only mated female adults undergo diapause, ...
ORGANISM(S): Bombus terrestris audax 
2019-11-18 | PXD014384 | Pride
Diapause Proteomics Amps Bombus 
ProteomicsThe Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic perspective
Aspergillus fumigatus and Pseudomonas aeruginosa are the most prevalent fungal and bacterial pathogens, associated with cystic fibrosis-related infections, respectively. Although it is well established that P. aeruginosa eventually predominates as the primary pathogen, it is unknown why this is the ...
ORGANISM(S): Homo sapiens (Human) 
2019-11-11 | PXD014022 | Pride
Lfq ms A549 cell line Aspergillus fumigatus Pseudomonas aeruginosa Cystic fibrosis 
ProteomicsCharacterization of the Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic Perspective
The following describes additional preliminary data to that outlined in Project PXD014022 and specifically describes the 12-hour co-exposure and P. aeruginosa 12 hour exposure preliminary experiments. Aspergillus fumigatus and Pseudomonas aeruginosa are the most prevalent fungal and bacterial patho...
ORGANISM(S): Homo sapiens (Human) 
2019-11-11 | PXD014535 | Pride
Aspergillus  cellular response  cystic fibrosis  fungal infection  pseudomonas 
ProteomicsThe Aspergillus fumigatus secretome alters the proteome of Pseudomonas aeruginosa to stimulate bacterial growth: implications for co-infection.
The airways of cystic fibrosis patients are chronically colonized by a diverse range of microbial pathogens, the composition of which changes throughout life Alteration to the pulmonary environment caused by inter-microbial interactions and pathogen-host interactions influence the type of microbes ...
ORGANISM(S): Pseudomonas aeruginosa 
2020-07-22 | PXD015056 | Pride
Pseudomonas  proteomics Lfq Aspergillus  cystic fibrosis 
ProteomicsA proteomic investigation of hepatic resistance to Ascaris in a murine model.
In 2010, Ascaris caused 819 million infections worldwide. The impact on children is particularly severe, causing growth retardation and detrimental effects on cognitive development. Transmission is linked to unhygienic defecation habits, making ascariasis a disease of poverty. The WHO recognises it ...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-08 | PXD003555 | Pride
Mouse Ascaris Lfq Liver 
ProteomicsThe effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis.
The large pine weevil Hylobius abietis L. is a major forestry pest in 15 European countries, where it is a threat to 3.4 million hectares of forest. A cellular and proteomic analysis of the effect of culture filtrate of three entomopathogenic fungi (EPF) species on the immune system of H. abietis wa...
ORGANISM(S): Hylobius abietis 
2018-07-23 | PXD008232 | Pride
Proteomics Entomopathogenic fungi Lfq Pine weevil Immune response 
ProteomicsThe liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response
Ascariasis is a neglected tropical disease that affects 800 million people worldwide. Whereas most people only experience light worm burden, some people experience heavy worm burdens even after several rounds of chemotherapy, a phenomenon known as predisposition. Such heavy infections are associated...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-12 | PXD014508 | Pride
Shotgun mass spectrometry Innate immune response Liver Mitochondria Proteome Ascaris 
ProteomicsThe Aspergillus fumigatus secretome alters the proteome of Pseudomonas aeruginosa to stimulate bacterial growth: implications for co-infection
Individuals with cystic fibrosis are susceptible to co-infection by Aspergillus fumigatus and Pseudomonas aeruginosa, however P. aeruginosa eventually predominates as the primary pathogen. Several factors are likely to facilitate P. aeruginosa colonization in the airways, including alterations to th...
ORGANISM(S): Aspergillus fumigatus Af293 
2020-07-22 | PXD018112 | Pride
Shotgun proteomics Lc-ms Culture filtrate Aspergillus fumigatus 
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